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Folding of Deoxymyoglobin Triggered by Electron Transfer

Wittung-Stafshede, Pernilla and Malmström, Bo G. and Winkler, Jay R. and Gray, Harry B. (1998) Folding of Deoxymyoglobin Triggered by Electron Transfer. Journal of Physical Chemistry A, 102 (28). pp. 5599-5601. ISSN 1089-5639. https://resolver.caltech.edu/CaltechAUTHORS:20180313-160333459

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Abstract

The met and deoxy forms of sperm whale myoglobin (Mb) can be unfolded by guanidine hydrochloride (GuHCl). Electronic absorption and circular dichroism spectroscopic measurements show that folded deoxyMb is more stable than the folded met protein. Laser excitation of NADH generates species that rapidly reduce unfolded metMb, triggering the formation of folded deoxyMb in less than 10 ms (pH 7.0, 2.5 to 3 M GuHCl, 20 °C). At comparable reaction driving forces (∼10 kJ/mol), deoxyMb folds much faster than reduced cytochrome c.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/jp9802228DOIArticle
https://pubs.acs.org/doi/abs/10.1021/jp9802228PublisherArticle
ORCID:
AuthorORCID
Wittung-Stafshede, Pernilla0000-0003-1058-1964
Winkler, Jay R.0000-0002-4453-9716
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1998 American Chemical Society. Received: November 17, 1997; In Final Form: April 1, 1998. Publication Date (Web): May 23, 1998. P.W.-S. thanks the Swedish Technical Research Council for a postdoctoral fellowship and Ivan Dmochowski for assistance with the diode array measurements. This work was supported by the National Science Foundation (H.B.G., J.R.W.), the Nobel Institute for Chemistry (B.G.M.), and the Arnold and Mabel Beckman Foundation.
Funders:
Funding AgencyGrant Number
Swedish Technical Research CouncilUNSPECIFIED
NSFUNSPECIFIED
Nobel Institute for ChemistryUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Issue or Number:28
Record Number:CaltechAUTHORS:20180313-160333459
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180313-160333459
Official Citation:Folding of Deoxymyoglobin Triggered by Electron Transfer. Pernilla Wittung-Stafshede, Bo G. Malmström, Jay R. Winkler, and, and Harry B. Gray. The Journal of Physical Chemistry A 1998 102 (28), 5599-5601. DOI: 10.1021/jp9802228
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85293
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Mar 2018 23:12
Last Modified:03 Oct 2019 19:29

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