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High-throughput ultraviolet photoacoustic microscopy with multifocal excitation

Imai, Toru and Shi, Junhui and Wong, Terence T. W. and Li, Lei and Zhu, Liren and Wang, Lihong V. (2018) High-throughput ultraviolet photoacoustic microscopy with multifocal excitation. Journal of Biomedical Optics, 23 (3). Art. No. 036007. ISSN 1083-3668. PMCID PMC5852316. doi:10.1117/1.JBO.23.3.036007.

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Ultraviolet photoacoustic microscopy (UV-PAM) is a promising intraoperative tool for surgical margin assessment (SMA), one that can provide label-free histology-like images with high resolution. In this study, using a microlens array and a one-dimensional (1-D) array ultrasonic transducer, we developed a high-throughput multifocal UV-PAM (MF-UV-PAM). Our new system achieved a 1.6  ±  0.2  μm lateral resolution and produced images 40 times faster than the previously developed point-by-point scanning UV-PAM. MF-UV-PAM provided a readily comprehensible photoacoustic image of a mouse brain slice with specific absorption contrast in ∼16  min, highlighting cell nuclei. Individual cell nuclei could be clearly resolved, showing its practical potential for intraoperative SMA.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Imai, Toru0000-0002-9497-8549
Shi, Junhui0000-0002-5741-2781
Wong, Terence T. W.0000-0001-6399-758X
Li, Lei0000-0001-6164-2646
Zhu, Liren0000-0002-6338-9338
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 170796R received Dec. 8, 2017; accepted for publication Feb. 22, 2018; published online Mar. 15, 2018. The authors appreciate the close reading of the manuscript by Prof. James Ballard. This work was sponsored by National Institutes of Health (NIH) Grants R01 CA186567 (NIH Director’s Transformative Research Award), U01 NS090579 (NIH BRAIN Initiative), U01 NS099717 (NIH BRAIN Initiative), and R01 EB016963, and supported in part by the March of Dimes Prematurity Research Center. L.V.W. has financial interests in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work.
Funding AgencyGrant Number
NIHR01 CA186567
NIHU01 NS090579
NIHU01 NS099717
NIHR01 EB016963
March of Dimes FoundationUNSPECIFIED
Subject Keywords:photoacoustic imaging; ultraviolet laser; surgical margin assessment; microlens array; 1-D array ultrasonic transducer
Issue or Number:3
PubMed Central ID:PMC5852316
Record Number:CaltechAUTHORS:20180319-130354510
Persistent URL:
Official Citation:Toru Imai, Junhui Shi, Terence T. W. Wong, Lei Li, Liren Zhu, Lihong V. Wang, "High-throughput ultraviolet photoacoustic microscopy with multifocal excitation," Journal of Biomedical Optics 23(3), 036007 (15 March 2018). Submission: Received 8 December 2017; Accepted 22 February 2018
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85357
Deposited By: Tony Diaz
Deposited On:26 Mar 2018 22:25
Last Modified:15 Nov 2021 20:28

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