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CRISPR Knockouts in Ciona Embryos

Gandhi, Shashank and Razy-Krajka, Florian and Christiaen, Lionel and Stolfi, Alberto (2018) CRISPR Knockouts in Ciona Embryos. In: Transgenic Ascidians. Advances in Experimental Medicine and Biology. No.1029. Springer , Singapore, pp. 141-152. ISBN 978-981-10-7544-5. http://resolver.caltech.edu/CaltechAUTHORS:20180319-130924469

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Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 has emerged as a revolutionary tool for fast and efficient targeted gene knockouts and genome editing in almost any organism. The laboratory model tunicate Ciona is no exception. Here, we describe our latest protocol for the design, implementation, and evaluation of successful CRISPR/Cas9-mediated gene knockouts in somatic cells of electroporated Ciona embryos. Using commercially available reagents, publicly accessible plasmids, and free web-based software applications, any Ciona researcher can easily knock out any gene of interest in their favorite embryonic cell lineage.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/978-981-10-7545-2_13DOIArticle
https://link.springer.com/chapter/10.1007%2F978-981-10-7545-2_13PublisherArticle
Additional Information:© 2018 Springer Nature Singapore Pte Ltd. First Online: 15 March 2018. Research in the laboratory of L.C. is supported by R01 awards HL108643 and GM096032 from the NIH/NHLBI and NIH/NIGMS respectively; and by grant 15CVD01 from the Leducq Foundation. A.S. is supported by R00 award HD084814 from the NIH/NICHD.
Funders:
Funding AgencyGrant Number
NIHHL108643
NIHGM096032
Leducq Foundation15CVD01
NIHHD084814
Subject Keywords:Genome editing; Targeted mutagenesis; Somatic gene knockout; sgRNAs; Tunicates; Chordates
Record Number:CaltechAUTHORS:20180319-130924469
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180319-130924469
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85358
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Mar 2018 22:22
Last Modified:26 Mar 2018 22:22

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