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The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha

Roginski, Raymond S. and Lau, Chi W. and Santoiemma, Phillip P. and Weaver, Sara J. and Du, Peicheng and Soteropoulos, Patricia and Yang, Jay (2018) The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha. Gene, 648 . pp. 42-53. ISSN 0378-1119. PMCID PMC6010073.

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The known functions of the human GCOM1 complex hub gene include transcription elongation and the intercalated disk of cardiac myocytes. However, in all likelihood, the gene's most interesting, and thus far least understood, roles will be found in the central nervous system. To investigate the functions of the GCOM1 gene in the CNS, we have cloned human and rat brain cDNAs encoding novel, 105 kDa GCOM1 combined (Gcom) proteins, designated Gcom15, and identified a new group of GCOM1 interacting genes, termed Gints, from yeast two-hybrid (Y2H) screens. We showed that Gcom15 interacts with the NR1 subunit of the NMDA receptor by co-expression in heterologous cells, in which we observed bi-directional co-immunoprecipitation of human Gcom15 and murine NR1. Our Y2H screens revealed 27 novel GCOM1 interacting genes, many of which are synaptic proteins and/or play roles in neurologic diseases. Finally, we showed, using rat brain protein preparations, that the Gint internexin-alpha (INA), a known interactor of the NMDAR, co-IPs with GCOM1 proteins, suggesting a GCOM1-GRIN1-INA interaction and a novel pathway that may be relevant to neuroprotection.

Item Type:Article
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Weaver, Sara J.0000-0001-7753-6215
Additional Information:© 2018 Published by Elsevier B.V. Received 14 February 2017, Accepted 6 January 2018, Available online 12 January 2018. DNA sequences deposited in GenBank: Human Gcom1, AY207007; human Gcom15, JF419331; rat Gcom15, JF440303. These studies were supported by: Education and Development funding from the Department of Anesthesiology and Critical Care of the University of Pennsylvania Perelman School of Medicine (2006-2009); a University of Pennsylvania Research Foundation award to RSR (2005-2006); a Department of Veterans Affairs Competitive Pilot Project Funding award to RSR (2013-2015); and NIH RO1 GM107054 to JY. Lee Vandevier received a stipend from the University of Pennsylvania's Provost Undergraduate Research Mentoring (PURM) Program in the summer of 2008 under the mentorship of RSR. The PURM Program also provided the PI with a small amount of funding for supplies. The mouse NR1-1a cDNA was a kind gift from R. S. Zukin, PhD. VA Disclaimer: The contents of this paper do not represent the views of the U.S. Department of Veterans Affairs or the United States Government.
Funding AgencyGrant Number
University of PennsylvaniaUNSPECIFIED
Department of Veterans AffairsUNSPECIFIED
NIHRO1 GM107054
Subject Keywords:GRIN1 gene; INA gene; Yeast two-hybrid; Gdown1/POLR2M; Gup1/MYZAP; Gcom15 protein
PubMed Central ID:PMC6010073
Record Number:CaltechAUTHORS:20180321-090741506
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Official Citation:Raymond S. Roginski, Chi W. Lau, Phillip P. Santoiemma, Sara J. Weaver, Peicheng Du, Patricia Soteropoulos, Jay Yang, The human GCOM1 complex gene interacts with the NMDA receptor and internexin-alpha, Gene, Volume 648, 2018, Pages 42-53, ISSN 0378-1119, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85390
Deposited By: Tony Diaz
Deposited On:26 Mar 2018 22:01
Last Modified:05 Mar 2020 19:59

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