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Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair

Ding, Yichen and Lee, Juhyun and Hsu, Jeffrey J. and Chang, Chih-Chiang and Baek, Kyung In and Ranjbarvazri, Sara and Ardehali, Reza and Sevag Packard, René R. and Hsiai, Tzung K. (2018) Light-Sheet Imaging to Elucidate Cardiovascular Injury and Repair. Current Cardiology Reports, 20 (5). Art. No. 35. ISSN 1523-3782.

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Purpose of Review: Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts. Recent Findings: Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. Summary: These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.

Item Type:Article
Related URLs:
URLURL TypeDescription ReadCube access
Ding, Yichen0000-0002-6242-3506
Hsu, Jeffrey J.0000-0002-9971-5916
Baek, Kyung In0000-0001-9388-2070
Ardehali, Reza0000-0003-1318-4016
Sevag Packard, René R.0000-0002-8520-5843
Hsiai, Tzung K.0000-0003-1734-0792
Additional Information:© 2018 Springer Science+Business Media, LLC, part of Springer Nature. First Online: 24 March 2018. The authors would like to express gratitude to all lab members for discussion and insights. Compliance with Ethical Standards. Conflict of Interest: Yichen Ding, Juhyun Lee, Jeffrey J. Hsu, Chih-Chiang Chang, Kyung In Baek, Sara Ranjbarvazirj, Reza Ardehali, René R. Sevag Packard, and Tzung K. Hsiai declare no conflict of interest. Human and Animal Rights and Informed Consent: This article does not contain any studies with human subjects performed by any of the authors. All animal rights have been approved by AAALAC and USDA.
Subject Keywords:Light-sheet imaging; Cardiovascular injury; Regeneration; Doxorubicin
Issue or Number:5
Record Number:CaltechAUTHORS:20180402-091245017
Persistent URL:
Official Citation:Ding, Y., Lee, J., Hsu, J.J. et al. Curr Cardiol Rep (2018) 20: 35.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85556
Deposited By: Tony Diaz
Deposited On:02 Apr 2018 16:37
Last Modified:03 Oct 2019 19:32

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