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Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging

Glass, Jennifer B. and Chen, Si and Dawson, Katherine S. and Horton, Damian R. and Vogt, Stefan and Ingall, Ellery D. and Twining, Benjamin S. and Orphan, Victoria J. (2017) Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging. Geomicrobiology Journal, 35 (1). pp. 81-89. ISSN 0149-0451. https://resolver.caltech.edu/CaltechAUTHORS:20180411-113723264

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Abstract

Metal cofactors are required for many enzymes in anaerobic microbial respiration. This study examined iron, cobalt, nickel, copper, and zinc in cellular and abiotic phases at the single-cell scale for a sulfate-reducing bacterium (Desulfococcus multivorans) and a methanogenic archaeon (Methanosarcina acetivorans) using synchrotron X-ray fluorescence microscopy. Relative abundances of cellular metals were also measured by inductively coupled plasma mass spectrometry. For both species, zinc and iron were consistently the most abundant cellular metals. M. acetivorans contained higher nickel and cobalt content than D. multivorans, likely due to elevated metal requirements for methylotrophic methanogenesis. Cocultures contained spheroid zinc sulfides and cobalt/copper sulfides.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1080/01490451.2017.1321068DOIArticle
https://www.tandfonline.com/doi/full/10.1080/01490451.2017.1321068PublisherArticle
https://doi.org/10.1101/087585DOIDiscussion Paper
ORCID:
AuthorORCID
Glass, Jennifer B.0000-0003-0775-2486
Chen, Si0000-0001-6619-2699
Dawson, Katherine S.0000-0001-8856-4609
Vogt, Stefan0000-0002-8034-5513
Ingall, Ellery D.0000-0003-1954-0317
Twining, Benjamin S.0000-0002-1365-9192
Orphan, Victoria J.0000-0002-5374-6178
Additional Information:© 2018 Taylor & Francis. Received 01 Nov 2016, Accepted 01 Apr 2017, Accepted author version posted online: 21 Apr 2017, Published online: 19 May 2017. This work was supported by a NASA Astrobiology Postdoctoral Fellowship to J.B.G, NASA Exobiology award NNX14AJ87G to J.B.G., DOE Biological and Environmental Research award DE-SC0004949 to V.J.O, NSF award OCE-0939564 to V.J.O, NSF award OCE-1232814 to B.S.T., and NSF award OCE-1357375 to E.D.I. This work was also supported by the NASA Astrobiology Institute Alternative Earths Team (Science Mission Directorate award NNA15BB03A). Use of the Advanced Photon Source, an Office of Science User Facility operated for the U.S. DOE Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02-06CH11357. Use of the LS-CAT Sector 21 was supported by the Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor (Grant 085P1000817). We thank two anonymous reviewers for helpful feedback on the previous version of this manuscript.
Funders:
Funding AgencyGrant Number
NASA Astrobiology Postdoctoral FellowshipUNSPECIFIED
NASANNX14AJ87G
Department of Energy (DOE)DE-SC0004949
NSFOCE-0939564
NSFOCE-1232814
NSFOCE-1357375
NASANNA15BB03A
Department of Energy (DOE)DE-AC02-06CH11357
Michigan Economic Development CorporationUNSPECIFIED
Michigan Technology Tri-Corridor085P1000817
Subject Keywords:Metals, methanogen, methanol, sulfate reduction, synchrotron X-ray fluorescence
Issue or Number:1
Record Number:CaltechAUTHORS:20180411-113723264
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180411-113723264
Official Citation:Jennifer B. Glass, Si Chen, Katherine S. Dawson, Damian R. Horton, Stefan Vogt, Ellery D. Ingall, Benjamin S. Twining & Victoria J. Orphan (2017) Trace Metal Imaging of Sulfate-Reducing Bacteria and Methanogenic Archaea at Single-Cell Resolution by Synchrotron X-Ray Fluorescence Imaging, Geomicrobiology Journal, 35:1, 81-89, DOI: 10.1080/01490451.2017.1321068
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:85749
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Apr 2018 19:08
Last Modified:03 Oct 2019 19:35

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