The process of infection with bacteriophage phiX174, XXII. Synthesis of progeny single-stranded DNA

Abstract

In a previous article,(1) it was demonstrated that of the labeled thymidine incorporated into the replicative form (RF) of phiX-DNA during the period of RF replication (3-12 or 15 min after infection), approximately half was transferred to virus during the subsequent period of single-strand DNA synthesis, while half remained in RF molecules. In this paper experiments are presented which permit a definition of the label remaining in the RF and which clarify the role of the RF in the synthesis of the progeny single-stranded DNA.