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Rearranged immunoglobulin heavy chain variable region (VH) pseudogene that deletes the second complementarity-determining region

Takahashi, Naoki and Noma, Takahumi and Honjo, Tasuku (1984) Rearranged immunoglobulin heavy chain variable region (VH) pseudogene that deletes the second complementarity-determining region. Proceedings of the National Academy of Sciences of the United States of America, 81 (16). pp. 5194-5198. ISSN 0027-8424. PMCID PMC391664. doi:10.1073/pnas.81.16.5194.

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We have cloned two rearranged heavy chain variable region (VH) genes from the IgG-producing human cell line CESS. The VH gene, which is linked to the µ chain constant region (Cµ) gene, has two deletions at residues 45-62 and 82A-90, the former of which corresponds closely to the second complementarity-determining region (CDR2). These results could indicate that translocation of CDR2 occured and could give support to the argument that reassortment of the V minigenes is involved in the generation of hypervariability during evolution. However, the rearranged pseudogene could have also arisen by fortuitous deletion. The other VH gene of CESS is an expressed form and is probably linked to the Cγ gene. The diversity region (D) segments used in these rearranged V genes are <38% homologous to known human germline D segments, indicating the presence of more unknown germline D segments.

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Additional Information:© 1984 by the National Academy of Sciences. Communicated by Elvin A. Kabat, March 30, 1984. We are grateful to Miss K. Kawabata for preparation of the manuscript. We thank Misses N. Kisogawa, Y. Utsunomiya, and K. Harato for their technical assistance. This work was supported by a grant-in-aid for special distinguished research of the Ministry of Education, Science and Culture of Japan. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
Subject Keywords:molecular cloning; DNA sequence determination; human VH gene
Issue or Number:16
PubMed Central ID:PMC391664
Record Number:CaltechAUTHORS:TAKpnas84
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8603
Deposited By: Tony Diaz
Deposited On:22 Aug 2007
Last Modified:08 Nov 2021 20:51

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