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Directed Evolution of Artificial Metalloenzymes: Bridging Synthetic Chemistry and Biology

Zhang, Ruijie K. and Romney, David K. and Kan, S. B. Jennifer and Arnold, Frances H. (2018) Directed Evolution of Artificial Metalloenzymes: Bridging Synthetic Chemistry and Biology. In: Artificial Metalloenzymes and MetalloDNAzymes in Catalysis. Wiley , Hoboken, NJ, pp. 137-170. ISBN 9783527341788. http://resolver.caltech.edu/CaltechAUTHORS:20180502-090421380

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Abstract

Directed evolution is a powerful algorithm for engineering proteins to have novel and useful properties. However, we do not yet fully understand the characteristics of an evolvable system. In this chapter, we present examples where directed evolution has been used to enhance the performance of metalloenzymes, focusing first on “classical” cases such as improving enzyme stability or expanding the scope of natural reactivity. We then discuss how directed evolution has been extended to artificial systems, in which a metalloprotein catalyzes reactions using abiological reagents or in which the protein utilizes a nonnatural cofactor for catalysis. These examples demonstrate that directed evolution can also be applied to artificial systems to improve catalytic properties, such as activity and enantioselectivity, and to favor a different product than that favored by small‐molecule catalysts. Future work will help define the extent to which artificial metalloenzymes can be altered and optimized by directed evolution and the best approaches for doing so.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/9783527804085.ch5DOIArticle
ORCID:
AuthorORCID
Romney, David K.0000-0003-0498-7597
Kan, S. B. Jennifer0000-0001-6371-8042
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2018 Wiley‐VCH Verlag GmbH & Co. KGaA. Published Online: 25 March 2018; Published Print: 14 May 2018.
Subject Keywords:directed evolution; fitness landscape; nonnatural reactivity; artificial metalloenzyme
Record Number:CaltechAUTHORS:20180502-090421380
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180502-090421380
Official Citation:Montserrat, D. , Jan‐E⋅, B. , Oscar, P. , Zhang, R. K., Romney, D. K., Kan, S. B. and Arnold, F. H. (2018). Directed Evolution of Artificial Metalloenzymes: Bridging Synthetic Chemistry and Biology. In Artificial Metalloenzymes and MetalloDNAzymes in Catalysis (eds D. Montserrat, B. Jan‐E⋅ and P. Oscar). doi:10.1002/9783527804085.ch5
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86189
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 May 2018 21:18
Last Modified:03 May 2018 21:18

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