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Dichroism-sensitive photoacoustic computed tomography

Qu, Yuan and Li, Lei and Shen, Yuecheng and Wei, Xiaoming and Wong, Terence T. W. and Hu, Peng and Yao, Junjie and Maslov, Konstantin and Wang, Lihong V. (2018) Dichroism-sensitive photoacoustic computed tomography. Optica, 5 (4). pp. 495-501. ISSN 2334-2536. PMCID PMC6388697. doi:10.1364/OPTICA.5.000495.

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Photoacoustic computed tomography (PACT), a fast-developing modality for deep tissue imaging, images the spatial distribution of optical absorption. PACT usually treats the absorption coefficient as a scalar. However, the absorption coefficients of many biological tissues exhibit an anisotropic property, known as dichroism or diattenuation, which depends on molecular conformation and structural alignment. Here, we present a novel imaging method called dichroism-sensitive PACT (DS-PACT), which measures both the amplitude of tissue’s dichroism and the orientation of the optic axis of uniaxial dichroic tissue. By modulating the polarization of linearly polarized light and measuring the alternating signals through lock-in detection, DS-PACT can boost dichroic signals from biological tissues. To validate the proposed approach, we experimentally demonstrated the performance of DS-PACT by imaging plastic polarizers and ex vivo bovine tendons deep inside scattering media. We successfully detected the orientation of the optic axis of uniaxial dichroic materials, even at a depth of 4.5 transport mean free paths. We anticipate that the proposed method will extend the capability of PACT to imaging tissue absorption anisotropy.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Li, Lei0000-0001-6164-2646
Shen, Yuecheng0000-0003-1990-8142
Wei, Xiaoming0000-0001-6136-5335
Wong, Terence T. W.0000-0001-6399-758X
Hu, Peng0000-0002-2933-1239
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. Received 16 March 2018; accepted 28 March 2018 (Doc. ID 325859); published 18 April 2018. We thank Yan Liu and Liang Gao for helpful discussions and sharing data. We thank Jinyang Liang, Yang Li, Hun He, and Bin Rao for their support in the experiment. We thank Professor James Ballard, Utku Baran, and Sandra Matteucci for proofreading the paper. This project is supported in part by the March of Dimes Prematurity Research Center. K. Maslov has a financial interest in Microphotoacoustics, Inc. L. V. Wang has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. Funding: National Institutes of Health (NIH) (DP1 EB016986, R01 CA186567, R01 EB016963, U01 NS090579, U01 NS099717); March of Dimes Foundation (3125-17303A).
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 EB016963
NIHU01 NS090579
NIHU01 NS099717
March of Dimes Foundation3125-17303A
Issue or Number:4
Classification Code:OCIS codes: (170.3880) Medical and biological imaging; (170.5120) Photoacoustic imaging; (100.1930) Dichroism; (290.5855) Scattering, polarization
PubMed Central ID:PMC6388697
Record Number:CaltechAUTHORS:20180509-110938282
Persistent URL:
Official Citation:Yuan Qu, Lei Li, Yuecheng Shen, Xiaoming Wei, Terence T. W. Wong, Peng Hu, Junjie Yao, Konstantin Maslov, and Lihong V. Wang, "Dichroism-sensitive photoacoustic computed tomography," Optica 5, 495-501 (2018)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86314
Deposited By: Tony Diaz
Deposited On:09 May 2018 20:52
Last Modified:11 Mar 2022 19:21

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