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Long-Range Structural Changes in the Meiotic Nucleus Revealed by Changes in Stress Communication Along the Chromosome

Newman, Trent and Beltran, Bruno G. and McGehee, James and Cahoon, Cori and Elnatan, Daniel and Chu, Daniel and Burgess, Sean and Spakowitz, Andrew (2018) Long-Range Structural Changes in the Meiotic Nucleus Revealed by Changes in Stress Communication Along the Chromosome. Biophysical Journal, 114 (3). 30a. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:20180510-092953077

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Abstract

Homologous recombination drives structural reorganization of the nucleus in early meiosis. In order to investigate the connection between homolog pairing, meiotic progression, and the dynamics of the underlying chromatin, we tracked flourescently labeled homolog pairs in synchronized S. cerevisae. Various previously unreported statistics of the anomalous inter-loci motion correlate with meiotic progression and can be quantitatively reproduced by a simple polymer model of the sister chromatids. The first of these is the distribution of waiting times for the homologous loci to come into and out of contact with each other (loosely, inter-locus “looping” and “unlooping” times). The full shape of the looping time distribution can be quantitatively reproduced by a simple model of two polymers diffusing independently in a spherical confinement. This finding suggests a dominant role for diffusion-limited, undirected search in homolog pairing in early meiosis. This is in sharp contrast with the intuition that a heavy-tailed search process could never drive such a critical cell-cycle stage. We further show that the inter-locus velocity-velocity correlation (VVC) quantitatively matches analytical results for the inter-locus VVC of our polymer model, allowing us to leverage our analytical theory to extract the time scale of stress communication between the labeled loci along the chromosome. We show that stresses can take tens of minutes to propagate between loci on paired chromosomes, and that the increasing connectivity between the chromosomes as the cell progresses through meiosis can be quantified by the shortening of this communication time. Our study highlights the power of coarse-grained polymer models to analyze dynamic structural properties of the nucleus in vivo and the importance of analytical theory for uncovering intracellular connections that might be obscured by lag times of many minutes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.bpj.2017.11.212DOIArticle
ORCID:
AuthorORCID
Spakowitz, Andrew0000-0002-0585-1942
Additional Information:© 2018 Elsevier Inc. Available online 6 February 2018.
Issue or Number:3
Record Number:CaltechAUTHORS:20180510-092953077
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180510-092953077
Official Citation:Long-Range Structural Changes in the Meiotic Nucleus Revealed by Changes in Stress Communication Along the Chromosome Newman, Trent et al. Biophysical Journal , Volume 114 , Issue 3 , 30a
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86332
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 May 2018 16:51
Last Modified:03 Oct 2019 19:42

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