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In vivo NMR analysis of the influence of pyruvate decarboxylase and alcohol dehydrogenase of Zymomonas mobilis on the anaerobic metabolism of Escherichia coli

Diaz Ricci, Juan C. and Hitzmann, Bernd and Bailey, James E. (1991) In vivo NMR analysis of the influence of pyruvate decarboxylase and alcohol dehydrogenase of Zymomonas mobilis on the anaerobic metabolism of Escherichia coli. Biotechnology Progress , 7 (4). pp. 305-310. ISSN 8756-7938. https://resolver.caltech.edu/CaltechAUTHORS:20180514-152001926

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Abstract

Phosphorus‐31 NMR studies of Escherichia coli expressing cloned pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) genes from Zymomonas mobilis revealed significant changes in the concentration of intracellular metabolites. The intracellular concentrations of phosphate, sugar phosphates, NAD(H), NTP, NDP, and UDPG of the strain HB101 expressing pdc, adh, or both genes were analyzed at quasi steady state during the anaerobic catabolism of glucose. The evolution of the intracellular pH and extracellular pH during glucose consumption showed a constant value of ΔpH equal to 0.2 pH unit that did not depend on the expression of the Zymomonas genes. The strains expressing PDC only exhibited greatest changes in intracellular composition with significantly decreased inorganic phosphate, NAD(H), NTP, and UDPG and significantly increased sugar phosphates. Analysis of the fermentation products during the NMR experiments provided further information about the physilogical changes observed in the cells. Two plasmid effects on the host metabolism are reported, one induced by the plasmid utilized as control (pUCl9), and the other induced by the expression of the pet operon. In both cases an enhancement of the glucose uptake rate was observed. Although the plasmid carrying adh induces a rapid metabolic inhibition, the expression of pdc does not. The expression of pdc and adh together showed a physiological enhancement that is attributable not to effects on external pH but rather to the effect of the switch in pathways from mixed acid to ethanol fermentation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/bp00010a003DOIArticle
Additional Information:© 1991 American Institute of Chemical Engineers (AIChE). Manuscript accepted: 27 May 1991. This research was supported by the National Science Foundation (Grant BCS-8912824). J.C.D.R. was supported in part by a fellowship from the Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina. B.H. was the recipient of a postdoctoral fellowship from the Deutscher Akademischer Austauschdienst (DAAD), FRG. NMR instrumentation employed in this research was supported in part by the Catalysis and Biocatalysis Program of the Advanced Industrial Concepts Division of DOE. L. O. Ingram kindly provided several plasmids employed in this research.
Funders:
Funding AgencyGrant Number
NSFBCS-8912824
Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)UNSPECIFIED
Deutscher Akademischer Austauschdienst (DAAD)UNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20180514-152001926
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180514-152001926
Official Citation:Ricci, J. C., Hitzmann, B. and Bailey, J. E. (1991), In Vivo NMR Analysis of the Influence of Pyruvate Decarboxylase and Alcohol Dehydrogenase of Zymomonas mobilis on the Anaerobic Metabolism of Escherichia coli. Biotechnol Progress, 7: 305-310. doi:10.1021/bp00010a003
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86396
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 May 2018 16:56
Last Modified:03 Oct 2019 19:43

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