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Design and Evaluation of a Peptidyl Fluorescent Chemosensor for Divalent Zinc

Walkup, Grant K. and Imperiali, Barbara (1996) Design and Evaluation of a Peptidyl Fluorescent Chemosensor for Divalent Zinc. Journal of the American Chemical Society, 118 (12). pp. 3053-3054. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20180515-155558284

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Abstract

The rapid analysis of trace metal cations for environmental and biomedical applications is particularly demanding since it requires the specific recognition of a particular element in the presence of numerous closely related species. While remarkable progress has been achieved for inorganic analytes, such as Ca^(2+), there is still a significant need for the genesis of new fluorosensors. In light of the selectivity and avidity with which proteins bind divalent metal cations, we have chosen to use a polypeptide architecture as the framework for metal ion recognition.In addition, our ability to manipulate synthetic polypeptides allows the coordinated integration of fluorescent reporters for signal transduction within a metal-binding peptidyl construct. Herein we report the design, synthesis, and evaluation of a selective fluorescent chemosensor, sensitive to nanomolar concentrations of Zn^(2+).


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/ja9538501DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/ja9538501PublisherSupporting Information
Additional Information:© 1996 American Chemical Society. Received November 16, 1995. Publication Date (Web): March 27, 1996. This research was supported by ONR, NSF, and the Caltech President's Fund. The award of a Glaxo Summer Fellowship (to G.K.W.) and a Camille and Henry Dreyfus Teacher Scholar Award (to B.I.) is also gratefully acknowledged.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
NSFUNSPECIFIED
Caltech President’s FundUNSPECIFIED
Glaxo Summer FellowshipUNSPECIFIED
Camille and Henry Dreyfus FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20180515-155558284
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180515-155558284
Official Citation:Design and Evaluation of a Peptidyl Fluorescent Chemosensor for Divalent Zinc. Grant K. Walkup and and Barbara Imperiali. Journal of the American Chemical Society 1996 118 (12), 3053-3054. DOI: 10.1021/ja9538501
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86417
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 May 2018 16:50
Last Modified:16 May 2018 16:50

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