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Rapid Formation of a Four-Helix Bundle. Cytochrome b_(562) Folding Triggered by Electron Transfer

Wittung-Stafshede, Pernilla and Gray, Harry B. and Winkler, Jay R. (1997) Rapid Formation of a Four-Helix Bundle. Cytochrome b_(562) Folding Triggered by Electron Transfer. Journal of the American Chemical Society, 119 (40). pp. 9562-9563. ISSN 0002-7863. doi:10.1021/ja971855n.

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Understanding how the secondary and tertiary structures of proteins are formed from non-native conformations is a continuing challenge for both theory and experiment. This complex process involves dynamics on time scales that range from picoseconds to minutes. We have shown previously that the folding of redox-active proteins can be triggered by electron transfer (ET), thereby opening the way for investigations of early events in the folding process. Since it is important that the redox-active cofactor remains bound to the unfolded protein (eliminating the possibility that the rate-limiting step will be bimolecular capture of the cofactor), we initially thought that only redox proteins with covalently attached cofactors would be amenable to study by this method. Indeed, thus far we have restricted our work on folding dynamics to ferrocytochrome c, a protein with a covalently attached heme.

Item Type:Article
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URLURL TypeDescription
Wittung-Stafshede, Pernilla0000-0003-1058-1964
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 1997 American Chemical Society. Received June 5, 1997. Publication Date (Web): October 8, 1997. Dedicated to Bo Malmström on the occasion of his 70th birthday. We thank S. Sligar (University of Illinois) for a generous gift of the plasmid containing the cytochrome b_(562) gene; N. Farrow for E. coli cells containing the plasmid as well as helpful discussions regarding purification; and J. Telford for assistance with the laser experiments. This work was supported by the Swedish Technical Research Council (PW-S) and the National Science Foundation (MCB-9630465).
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Swedish Technical Research CouncilUNSPECIFIED
Issue or Number:40
Record Number:CaltechAUTHORS:20180517-150025275
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Official Citation:Rapid Formation of a Four-Helix Bundle. Cytochrome b562 Folding Triggered by Electron Transfer. Pernilla Wittung-Stafshede, Harry B. Gray, and Jay R. Winkler. Journal of the American Chemical Society 1997 119 (40), 9562-9563. DOI: 10.1021/ja971855n
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86442
Deposited By: Tony Diaz
Deposited On:18 May 2018 16:47
Last Modified:15 Nov 2021 20:38

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