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Modification of Heme Peptides by Reverse Proteolysis:  Spectroscopy of Microperoxidase-10 with C-Terminal Histidine, Tyrosine, and Methionine Residues

Low, Donald W. and Yang, Grace and Winkler, Jay R. and Gray, Harry B. (1997) Modification of Heme Peptides by Reverse Proteolysis:  Spectroscopy of Microperoxidase-10 with C-Terminal Histidine, Tyrosine, and Methionine Residues. Journal of the American Chemical Society, 119 (17). pp. 4094-4095. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20180517-161822956

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Abstract

Small-molecule analogs of the active sites of heme proteins are under investigation in many laboratories. Covalent attachment of ligands to the porphyrins is often desirable as it reduces complications due to axial ligand dissociation and scrambling in cases involving mixed axial ligation. The preparation of ligand-linked (tailed) porphyrin systems, however, often involves complex multistep syntheses. We have found that trypsin-catalyzed reverse proteolysis provides a simple preparative route to a novel class of water-soluble tailed porphyrins based on the well-characterized microperoxidase (MP) framework. Using reverse proteolysis reactions, we have obtained mutant microperoxidase decapeptides (MP10s) with C-terminal histidine (H23MP10), tyrosine (Y23MP10), and methionine (M23MP10) residues. In addition, we have investigated the electronic absorption and resonance Raman spectra of these MP10 mutants.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/ja970103qDOIArticle
https://pubs.acs.org/doi/suppl/10.1021/ja970103qPublisherSupporting Information
ORCID:
AuthorORCID
Winkler, Jay R.0000-0002-4453-9716
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 1997 American Chemical Society. Received January 15, 1997. Publication Date (Web): April 30, 1997. We thank Adrian Ponce for technical assistance and John Dawson for helpful discussions. D.W.L. acknowledges a fellowship from the Parsons Foundation. This work was supported by the NSF.
Funders:
Funding AgencyGrant Number
Ralph M. Parsons FoundationUNSPECIFIED
NSFUNSPECIFIED
Issue or Number:17
Record Number:CaltechAUTHORS:20180517-161822956
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180517-161822956
Official Citation:Modification of Heme Peptides by Reverse Proteolysis:  Spectroscopy of Microperoxidase-10 with C-Terminal Histidine, Tyrosine, and Methionine Residues. Donald W. Low, Grace Yang, Jay R. Winkler, and Harry B. Gray. Journal of the American Chemical Society 1997 119 (17), 4094-4095. DOI: 10.1021/ja970103q
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86445
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 May 2018 16:52
Last Modified:22 Nov 2019 09:58

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