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Glutamine Phosphoribosylpyrophosphate Amidotransferase-independent Phosphoribosyl Amine Synthesis from Ribose 5-Phosphate and Glutamine or Asparagine

Koenigsknecht, Mark J. and Ramos, Itzel and Downs, Diana M. (2007) Glutamine Phosphoribosylpyrophosphate Amidotransferase-independent Phosphoribosyl Amine Synthesis from Ribose 5-Phosphate and Glutamine or Asparagine. Journal of Biological Chemistry, 282 (39). pp. 28379-28384. ISSN 0021-9258. doi:10.1074/jbc.M704024200. https://resolver.caltech.edu/CaltechAUTHORS:KOEjbc07

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Abstract

Phosphoribosylamine (PRA) is the first intermediate in the common pathway to purines and thiamine and is generated in bacteria by glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase (EC 2.4.2.14) from PRPP and glutamine. Genetic data have indicated that multiple, non-PRPP amidotransferase mechanisms exist to generate PRA sufficient for thiamine but not purine synthesis. Here we describe the purification and identification of an activity (present in both Escherichia coli and Salmonella enterica) that synthesizes PRA from ribose 5-phosphate and glutamine/asparagine. A purification resulting in greater than a 625-fold increase in specific activity identified 8 candidate proteins. Of the candidates, overexpression of AphA (EC 3.1.3.2), a periplasmic class B nonspecific acid phosphatase, significantly increased activity in partially purified extracts. Native purification of AphA to >95% homogeneity determined that the periplasmic L-asparaginase II, AnsB (EC 3.5.1.1), co-purified with AphA and was also necessary for PRA formation. The potential physiological relevance of AphA and AnsB in contributing to thiamine biosynthesis in vivo is discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dx.doi.org/10.1074/jbc.M704024200DOIArticle
http://www.jbc.org/content/282/39/28379.longPublisherArticle
Additional Information:© 2007 the American Society for Biochemistry and Molecular Biology. Submitted on May 16, 2007. Revised on July 23, 2007. Accepted on August 8, 2007. Papers In Press, published online ahead of print August 8, 2007 This work was supported by competitive grant GM47296 from the NIH. Funds were also provided from a 21st Century Scientist Scholars Award from the J.S. McDonnell Foundation. I. Ramos was supported by a pre-doctoral fellowship from CONACyT (Mexico). We acknowledge Eugenio I. Vivas for his assistance in the cloning and purification of PurD and John Roth for his generosity in supplying the aphA15::Cm allele.
Funders:
Funding AgencyGrant Number
NIHGM47296
James S. McDonnell FoundationUNSPECIFIED
Consejo Nacional de Ciencia y Tecnología (CONACYT)UNSPECIFIED
Issue or Number:39
DOI:10.1074/jbc.M704024200
Record Number:CaltechAUTHORS:KOEjbc07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KOEjbc07
Official Citation:Mark J. Koenigsknecht, Itzel Ramos, and Diana M. Downs Glutamine Phosphoribosylpyrophosphate Amidotransferase-independent Phosphoribosyl Amine Synthesis from Ribose 5-Phosphate and Glutamine or Asparagine J. Biol. Chem. 2007 282: 28379-. doi:10.1074/jbc.M704024200
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8654
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Sep 2007
Last Modified:08 Nov 2021 20:52

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