Diaz-Ricci, Juan C. and Hitzmann, Bernd and Rinas, Ursula and Bailey, James E. (1990) Comparative studies of glucose catabolism by Escherichia coli grown in a complex medium under aerobic and anaerobic conditions. Biotechnology Progress, 6 (5). pp. 326-332. ISSN 8756-7938. doi:10.1021/bp00005a003. https://resolver.caltech.edu/CaltechAUTHORS:20180524-152033875
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Abstract
Escherichia coli HB101 was grown in complex medium under anaerobic and aerobic conditions. Cells prepared under these two different conditions were characterized by two-dimensional protein gel electrophoresis, by NMR measurements under identical (anaerobic) conditions, and by measuring the kinetics of glucose uptake and catabolite end-product appearance in the medium under identical anaerobic conditions. Specific rates of glucose uptake and end-product formation were significantly greater for the anaerobically grown cells, which also exhibited lower intracellular concentrations of sugar phosphates, nucleoside di- and triphosphates, UDPG, and NAD(H). Two-dimensional gel electrophoretic analyses reveal changes in the intracellular levels of proteins involved in pyruvate catabolism that have been observed previously for E. coli grown in minimal medium under aerobic and anaerobic conditions. Enzymes involved in the TCA cycle were not detected in cells grown aerobically or anaerobically in complex medium.
Item Type: | Article | ||||||||||
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Additional Information: | © 1990 American Chemical Society and American Institute of Chemical Engineers. Accepted July 10, 1990. This research was supported by the Energy Conservation and Utilization Technologies (ECUT) Program of the U.S. Department of Energy and by the National Science Foundation (Grant BCS-8912824). J.C.D.-R. was supported by a postdoctoral fellowship from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. B.H. and U.R. were recipents of postdoctoral fellowships from the Deutscher Akademischer Austauschdienst (DAAD), FRG. | ||||||||||
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Issue or Number: | 5 | ||||||||||
DOI: | 10.1021/bp00005a003 | ||||||||||
Record Number: | CaltechAUTHORS:20180524-152033875 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180524-152033875 | ||||||||||
Official Citation: | Comparative studies of glucose catabolism by Escherichia coli grown in a complex medium under aerobic and anaerobic conditions Juan C. Diaz-Ricci, Bernd Hitzmann, Ursula Rinas, and James E. Bailey Biotechnology Progress 1990 6 (5), 326-332 DOI: 10.1021/bp00005a003 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 86598 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | George Porter | ||||||||||
Deposited On: | 24 May 2018 22:32 | ||||||||||
Last Modified: | 15 Nov 2021 20:40 |
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