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Environment of the Heme in Myoglobins. NMRD and EPR Spectroscopy of Val68X (X = Asn, Asp, and Glu) Mutants of Human Myoglobin

Zewert, Thomas E. and Gray, Harry B. and Bertini, Ivano (1994) Environment of the Heme in Myoglobins. NMRD and EPR Spectroscopy of Val68X (X = Asn, Asp, and Glu) Mutants of Human Myoglobin. Journal of the American Chemical Society, 116 (4). pp. 1169-1173. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20180529-152346680

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Abstract

Analyses of the temperature dependences and shapes of nuclear magnetic relaxation dispersion (NMRD) signals of site-directed mutants of human myoglobin indicate that a water molecule is bound to the sixth coordination site of the ferric heme in proteins in which the valine at position 68 is changed to either aspartate (Val68Asp) or asparagine (Val68Asn). Both NMRD data and electron paramagetic resonance (EPR) spectra show that carboxylate is axially ligated to Fe(III) in the Va168Glu mutant. The EPR spectra of the Va168Asp and Va168Asn derivatives show much smaller rhombic splittings than the spectrum of the Va168Glu protein.


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https://dx.doi.org/10.1021/ja00083a001DOIArticle
Additional Information:© 1994 American Chemical Society. Received August 23, 1993. We thank Seymour Koenig and Rodney Brown of the IBM Thomas J. Watson Research Center for allowing us access to their facilities and for assistance with many of the experiments. During the course of the work, we enjoyed several discussions with Claudio Luchinat, Lucia Banci, and Paul Smith. Research at Caltech was supported by the National Science Foundation, the National Institutes of Health, and the Arnold and Mabel Beckman Foundation.
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Record Number:CaltechAUTHORS:20180529-152346680
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180529-152346680
Official Citation:Environment of the Heme in Myoglobins. NMRD and EPR Spectroscopy of Val68X (X = Asn, Asp, and Glu) Mutants of Human Myoglobin Thomas E. Zewert, Harry B. Gray, and Ivano Bertini Journal of the American Chemical Society 1994 116 (4), 1169-1173 DOI: 10.1021/ja00083a001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86685
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:29 May 2018 23:08
Last Modified:03 Oct 2019 19:46

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