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Fluctuations in closed-loop fluorescent particle tracking

Berglund, Andrew J. and McHale, Kevin and Mabuchi, Hideo (2007) Fluctuations in closed-loop fluorescent particle tracking. Optics Express, 15 (12). pp. 7752-7773. ISSN 1094-4087.

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We present a comprehensive theory of closed-loop particle tracking for calculating the statistics of a diffusing fluorescent particle’s motion relative to the tracking lock point. A detailed comparison is made between the theory and experimental results, with excellent quantitative agreement found in all cases. A generalization of the theory of (open-loop) fluorescence correlation spectroscopy is developed, and the relationship to previous results is discussed. Two applications of the statistical techniques are given: a method for determining a tracked particle’s localization and an algorithm for rapid particle classification based on real-time analysis of the tracking control signal.

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Additional Information:© 2007 Optical Society of America Received 4 May 2007; revised 6 June 2007; accepted 6 June 2007; published 7 June 2007. K. McHale acknowledges a National Defense Science and Engineering Graduate fellowship. This work was supported by the National Science Foundation under grant CCF-0323542 and by the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the Army Research Office. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors, and do not necessarily reflect the views of either funding agency.
Subject Keywords:Fluorescence microscopy, Scanning microscopy, Fluorescence Spectroscopy
Issue or Number:12
Record Number:CaltechAUTHORS:BERoe07
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Official Citation:A. J. Berglund, K. McHale, and H. Mabuchi, "Fluctuations in closed-loop fluorescent particle tracking," Opt. Express 15, 7752-7773 (2007)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8674
Deposited By: Archive Administrator
Deposited On:04 Sep 2007
Last Modified:02 Oct 2019 23:53

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