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Structural and Functional Characterization of a Constrained Asx-Turn Motif

Imperiali, Barbara and Spencer, Jeffrey R. and Struthers, Mary D. (1994) Structural and Functional Characterization of a Constrained Asx-Turn Motif. Journal of the American Chemical Society, 116 (18). pp. 8424-8425. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20180601-162823795

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Abstract

The specific and timely glycosylation of proteins is associated with the proper structure, function, and targeting of glycoprotein conjugates. For N-linked glycosylation, catalyzed by the enzyme oligosaccharyl transferase (OT), we have recently proposed that the fidelity of this modification may be associated with particular conformational tendencies of polypeptide substrates which contain the consensus sequence Asn-Xaa-Thr/Ser. The presence of a local Asx-turn ,about the Asn-Xaa-Thr/Ser sequence, is central to this proposal, which was based on studies of the glycosyl acceptor properties of linear and cyclic peptides. Herein, we describe a new substrate for N-linked glycosylation which is conformationally constrained to adopt an Asx-turn and specifically designed to test this proposal.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dx.doi.org/10.1021/ja00097a083DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/ja00097a083PublisherSupporting Information
Additional Information:© 1994 American Chemical Society. Received June 7, 1994. This research was supported by National Institutes of Health Grant GM39334 and an NSF predoctoral fellowship to M.D.S. B.I. also gratefully acknowledges support from the Alfred P. Sloan Foundation, the Camille and Henry Dreyfus Teacher-Scholar Program, and Zeneca Inc. We acknowledge the Dorothy Chandler, Camilla Chandler Frost Laboratory in Biology at Caltech for use of NMR instrumentation (Varian Unity Plus 600 Spectrometer).
Funders:
Funding AgencyGrant Number
NIHGM39334
NSF Predoctoral FellowshipUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Camille and Henry Dreyfus FoundationUNSPECIFIED
ZenecaUNSPECIFIED
Issue or Number:18
Record Number:CaltechAUTHORS:20180601-162823795
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180601-162823795
Official Citation:Structural and Functional Characterization of a Constrained Asx-Turn Motif Barbara Imperiali, Jeffrey R. Spencer, and Mary D. Struthers Journal of the American Chemical Society 1994 116 (18), 8424-8425 DOI: 10.1021/ja00097a083
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86767
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Jun 2018 15:19
Last Modified:03 Oct 2019 19:48

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