CaltechAUTHORS
  A Caltech Library Service

Conformations of the single-stranded DNA of bacteriophage M13

Forsheit, Arleen B. and Ray, Dan S. (1970) Conformations of the single-stranded DNA of bacteriophage M13. Proceedings of the National Academy of Sciences of the United States of America, 67 (3). pp. 1534-1541. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:FORpnas70

[img]
Preview
PDF
See Usage Policy.

1409Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:FORpnas70

Abstract

At least two conformations of M13 single-stranded DNA have been demonstrated by measuring differences in sedimentation coefficient and by direct visualization in the electron microscope. Which form is obtained from infected cells and/or intact phage depends on the pH, ionic strength, and temperature. The slower-sedimenting form can be converted to the faster-sedimenting, single-stranded form by low ionic strength, alkali treatment, formamide, or formaldehyde, but not by exposure to 100 degrees C in 1.0 M NaCl. The ability to assume either conformation appears to be a function of the nucleic acid alone. Whether or not these different conformations are of biological significance is still unknown.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.pnas.org/cgi/content/abstract/67/3/1534OtherUNSPECIFIED
Additional Information:© 1970 by the National Academy of Sciences. Communicated by Norman Davidson, August 28, 1970. Supported by NSF grant GB 18074, University of California Cancer Research Funds, and an NSF predoctoral traineeship to A.B.F.
Issue or Number:3
Record Number:CaltechAUTHORS:FORpnas70
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:FORpnas70
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8680
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Sep 2007
Last Modified:02 Oct 2019 23:53

Repository Staff Only: item control page