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cis-acting mutations that affect rop protein control of plasmid copy number

Moser, David R. and Ma, Doreen and Moser, Catherine D. and Campbell, Judith L. (1984) cis-acting mutations that affect rop protein control of plasmid copy number. Proceedings of the National Academy of Sciences of the United States of America, 81 (14). pp. 4465-4469. ISSN 0027-8424.

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A number of pMB1 derivatives provide a trans-acting function that can suppress lethal runaway replication of a temperature-sensitive copy-number mutant of NTP1. Deletion analysis indicates that the region of the pMB1 genome that contains the rop gene is required for this suppression. Mutant derivatives of the temperature-sensitive copy-number mutant plasmid whose conditional lethal phenotype is not suppressed in trans by the region encoding the rop gene have been isolated. These rop-insensitive derivatives contain single nucleotide changes within the RNA I coding region.

Item Type:Article
Additional Information:© 1984 by the National Academy of Sciences. Communicated by Charles C. Richardson, March 20, 1984. This investigation was supported by Public Health Service Grant GM 25508 and Research Career Development Award CA 00544 to J.L.C. from the National Institutes of Health and by Public Health Service National Research Service Award Training Grant 5 T32 GM07616 to D.R.M. from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:regulation of DNA replication; temperature-sensitive mutant plasmid; small plasmid-encoded protein
Issue or Number:14
Record Number:CaltechAUTHORS:MOSpnas84
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8681
Deposited By: Tony Diaz
Deposited On:06 Sep 2007
Last Modified:02 Oct 2019 23:53

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