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Vibrational imaging of glucose uptake activity in live cells and tissues by stimulated Raman scattering microscopy

Hu, Fanhao and Chen, Zhixing and Zhang, Luyuan and Shen, Yuhui and Wei, Lu and Min, Wei (2016) Vibrational imaging of glucose uptake activity in live cells and tissues by stimulated Raman scattering microscopy. In: Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII. Proceedings of SPIE. No.9723. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 97230B. ISBN 9781628419573. http://resolver.caltech.edu/CaltechAUTHORS:20180608-114633492

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Abstract

Glucose is consumed as an energy source by virtually all living organisms, from bacteria to humans. Its uptake activity closely reflects the cellular metabolic status in various pathophysiological transformations, such as diabetes and cancer. Extensive efforts such as positron emission tomography, magnetic resonance imaging and fluorescence microscopy have been made to specifically image glucose uptake activity but all with technical limitations. Here, we report a new platform to visualize glucose uptake activity in live cells and tissues with subcellular resolution and minimal perturbation. A novel glucose analogue with a small alkyne tag (carbon-carbon triple bond) is developed to mimic natural glucose for cellular uptake, which can be imaged with high sensitivity and specificity by targeting the strong and characteristic alkyne vibration on stimulated Raman scattering (SRS) microscope to generate a quantitative three dimensional concentration map. Cancer cells with differing metabolic characteristics can be distinguished. Heterogeneous uptake patterns are observed in tumor xenograft tissues, neuronal culture and mouse brain tissues with clear cell-cell variations. Therefore, by offering the distinct advantage of optical resolution but without the undesirable influence of bulky fluorophores, our method of coupling SRS with alkyne labeled glucose will be an attractive tool to study energy demands of living systems at the single cell level.


Item Type:Book Section
Related URLs:
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http://dx.doi.org/10.1117/12.2211787.4848770176001DOIArticle
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).
Record Number:CaltechAUTHORS:20180608-114633492
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180608-114633492
Official Citation:Fanghao Hu, Zhixing Chen, Luyuan Zhang, Yihui Shen, Lu Wei, Wei Min, "Vibrational imaging of glucose uptake activity in live cells and tissues by stimulated Raman scattering microscopy (Conference Presentation)", Proc. SPIE 9723, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VIII, 97230B (27 April 2016); doi: 10.1117/12.2211787; https://doi.org/10.1117/12.2211787
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86921
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Jun 2018 23:47
Last Modified:08 Jun 2018 23:47

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