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Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering

Hu, Fanghao and Chen, Zhixing and Zhang, Luyuan and Shen, Yihui and Wei, Lu and Min, Wei (2015) Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering. Angewandte Chemie International Edition, 54 (34). pp. 9821-9825. ISSN 1433-7851. PMCID PMC4644272. https://resolver.caltech.edu/CaltechAUTHORS:20180608-124956778

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Abstract

Glucose is a ubiquitous energy source for most living organisms. Its uptake activity closely reflects cellular metabolic demand in various physiopathological conditions. Extensive efforts have been made to specifically image glucose uptake, such as with positron emission tomography, magnetic resonance imaging, and fluorescence microscopy, but all have limitations. A new platform to visualize glucose uptake activity in live cells and tissues is presented that involves performing stimulated Raman scattering on a novel glucose analogue labeled with a small alkyne moiety. Cancer cells with differing metabolic activities can be distinguished. Heterogeneous uptake patterns are observed with clear cell-cell variations in tumor xenograft tissues, neuronal culture, and mouse brain tissues. By offering the distinct advantage of optical resolution but without the undesirable influence of fluorophores, this method will facilitate the study of energy demands of living systems with subcellular resolution.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/anie.201502543DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4644272/PubMed CentralArticle
ORCID:
AuthorORCID
Wei, Lu0000-0001-9170-2283
Min, Wei0000-0003-2570-3557
Additional Information:© 2015 WILEY‐VCH. Received: March 18, 2015. Revised: April 26, 2015. Published online: July 16, 2015. We thank Y. Shin for providing hippocampal neurons and J. Hirtz for assistance on mouse brain tissues. W.M. acknowledges support from the NIH Director’s New Innovator Award, ARO MURI W911NF-12-1-0594, and a Alfred P. Sloan Research Fellowship.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Army Research Office (ARO)W911NF-12-1-0594
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:alkyne tags · glucose uptake · imaging agents · Raman spectroscopy · SRS microscopy
Issue or Number:34
PubMed Central ID:PMC4644272
Record Number:CaltechAUTHORS:20180608-124956778
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180608-124956778
Official Citation:Hu, F. , Chen, Z. , Zhang, L. , Shen, Y. , Wei, L. and Min, W. (2015), Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering. Angew. Chem. Int. Ed., 54: 9821-9825. doi:10.1002/anie.201502543
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86923
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:11 Jun 2018 18:06
Last Modified:23 Oct 2019 20:54

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