Hu, Fanghao and Zeng, Chen and Long, Rong and Miao, Yupeng and Wei, Lu and Xu, Qizhi and Min, Wei (2018) Supermultiplexed optical imaging and barcoding with engineered polyynes. Nature Methods, 15 (3). pp. 194-200. ISSN 1548-7091. PMCID PMC5831481. https://resolver.caltech.edu/CaltechAUTHORS:20180608-125759681
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20180608-125759681
Abstract
Optical multiplexing has a large impact in photonics, the life sciences and biomedicine. However, current technology is limited by a 'multiplexing ceiling' from existing optical materials. Here we engineered a class of polyyne-based materials for optical supermultiplexing. We achieved 20 distinct Raman frequencies, as 'Carbon rainbow', through rational engineering of conjugation length, bond-selective isotope doping and end-capping substitution of polyynes. With further probe functionalization, we demonstrated ten-color organelle imaging in individual living cells with high specificity, sensitivity and photostability. Moreover, we realized optical data storage and identification by combinatorial barcoding, yielding to our knowledge the largest number of distinct spectral barcodes to date. Therefore, these polyynes hold great promise in live-cell imaging and sorting as well as in high-throughput diagnostics and screening.
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Additional Information: | © 2018 Macmillan Publishers Limited. Received: 23 August 2017. Accepted: 05 December 2017. Published: 15 January 2018. We are grateful for the discussion with L. Brus, Y. Shen and Z. Chen. W.M. acknowledges support from NIH Director's New Innovator Award (1DP2EB016573), R01 (EB020892), the US Army Research Office (W911NF-12-1-0594), and the Camille and Henry Dreyfus Foundation. Author Contributions: F.H. performed the spectroscopy, microscopy and biological studies and analyzed the data with the help of Y.M., L.W. and Q.X.; C.Z. performed the chemical synthesis together with R.L.; F.H. and W.M. conceived the concept; F.H., C.Z. and W.M. designed the experiments and wrote the manuscript with input from all authors. Competing interests: Columbia University has filed a patent application (US 62/540,953) based on this study. Code availability. The MATLAB code is available from the corresponding author upon request. Life Sciences Reporting Summary. Further information on experimental design is available in the Life Sciences Reporting Summary. Data availability. The data that support the findings of this study are provided in Supplementary Figures 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, Supplementary Tables 1–3 and Supplementary Note 1 and are available from the corresponding author upon request. | ||||||||||||
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Issue or Number: | 3 | ||||||||||||
PubMed Central ID: | PMC5831481 | ||||||||||||
Record Number: | CaltechAUTHORS:20180608-125759681 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180608-125759681 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 86924 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | George Porter | ||||||||||||
Deposited On: | 08 Jun 2018 20:40 | ||||||||||||
Last Modified: | 09 Mar 2020 13:19 |
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