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Live-cell quantitative imaging of proteome degradation by stimulated Raman scattering

Shen, Yihui and Xu, Fang and Wei, Lu and Hu, Fanghao and Min, Wei (2014) Live-cell quantitative imaging of proteome degradation by stimulated Raman scattering. Angewandte Chemie International Edition, 53 (22). pp. 5596-5599. ISSN 1433-7851. PMCID PMC4231775. https://resolver.caltech.edu/CaltechAUTHORS:20180608-133129304

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Abstract

Protein degradation is a regulatory process essential to cell viability and its dysfunction is implicated in many diseases, such as aging and neurodegeneration. In this report, stimulated Raman scattering microscopy coupled with metabolic labeling with ^(13)C-phenylalanine is used to visualize protein degradation in living cells with subcellular resolution. We choose the ring breathing modes of endogenous ^(12)C-phenylalanine and incorporated ^(13)C-phenylalanine as protein markers for the original and nascent proteomes, respectively, and the decay of the former wasquantified through ^(12)C/(^(12)C + ^(13)C) ratio maps. We demonstrate time-dependent imaging of proteomic degradation in mammalian cells under steady-state conditions and various perturbations, including oxidative stress, cell differentiation, and huntingtin protein aggregation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/anie.201310725DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231775/PubMed CentralArticle
ORCID:
AuthorORCID
Wei, Lu0000-0001-9170-2283
Additional Information:© 2014 WILEY‐VCH. Received: December 10, 2013. Published online: April 15, 2014. W.M. acknowledges support from National Institutes of Health Director’s New Innovator Award and Sloan Research Fellowship.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Subject Keywords:Raman spectroscopy; SRS microscopy; isotope labeling; protein aggregation; protein degradation
Issue or Number:22
PubMed Central ID:PMC4231775
Record Number:CaltechAUTHORS:20180608-133129304
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180608-133129304
Official Citation:Shen, Y. , Xu, F. , Wei, L. , Hu, F. and Min, W. (2014), Live‐Cell Quantitative Imaging of Proteome Degradation by Stimulated Raman Scattering. Angew. Chem. Int. Ed., 53: 5596-5599. doi:10.1002/anie.201310725
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86927
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:08 Jun 2018 22:38
Last Modified:23 Oct 2019 20:52

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