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Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

Wei, Lu and Hu, Fanghao and Shen, Yihui and Chen, Zhixing and Yu, Yong and Lin, Chih-Chun and Wang, Meng C. and Min, Wei (2014) Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering. Nature Methods, 11 (4). pp. 410-412. ISSN 1548-7091. PMCID PMC4040164.

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Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

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URLURL TypeDescription CentralArticle ReadCube access
Wei, Lu0000-0001-9170-2283
Hu, Fanghao0000-0002-8659-4027
Min, Wei0000-0003-2570-3557
Additional Information:© 2014 Macmillan Publishers Limited. Received 2 October 2013; accepted 29 January 2014; published online 2 March 2014. We thank L. Zhang, L. Brus, V.W. Cornish, D. Peterka and R. Yuste for helpful discussions. We are grateful to Y. Shin and X. Gao for technical assistance. W.M. acknowledges support from Columbia University, a US National Institutes of Health Director's New Innovator Award, the US Army Research Office (W911NF-12-1-0594) and an Alfred P. Sloan Research Fellowship. Author Contributions: L.W., F.H., Y.S., Z.C., Y.Y., C.-C.L. and M.C.W. performed experiments and analyzed data. L.W. and W.M. conceived the concept, designed the experiments and wrote the paper. Competing interests: Columbia University, which L.W., F.H., Y.S., Z.C. and W.M. are affiliated with, has filed a patent application based on this work.
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Columbia UniversityUNSPECIFIED
Army Research Office (ARO)W911NF-12-1-0594
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:4
PubMed Central ID:PMC4040164
Record Number:CaltechAUTHORS:20180608-133705699
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86929
Deposited By: George Porter
Deposited On:08 Jun 2018 22:34
Last Modified:09 Mar 2020 13:19

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