Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering

Wei, Lu and Hu, Fanghao and Shen, Yihui and Chen, Zhixing and Yu, Yong and Lin, Chih-Chun and Wang, Meng C. and Min, Wei (2014) Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering. Nature Methods, 11 (4). pp. 410-412. ISSN 1548-7091. PMCID PMC4040164. https://resolver.caltech.edu/CaltechAUTHORS:20180608-133705699

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Abstract

Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

Item Type:

Article

Related URLs:

URL	URL Type	Description
https://doi.org/10.1038/nmeth.2878	DOI	Article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040164/	PubMed Central	Article
https://rdcu.be/URNO	Publisher	Free ReadCube access

ORCID:

Author	ORCID
Wei, Lu	0000-0001-9170-2283
Hu, Fanghao	0000-0002-8659-4027
Min, Wei	0000-0003-2570-3557

Additional Information:

© 2014 Macmillan Publishers Limited. Received 2 October 2013; accepted 29 January 2014; published online 2 March 2014. We thank L. Zhang, L. Brus, V.W. Cornish, D. Peterka and R. Yuste for helpful discussions. We are grateful to Y. Shin and X. Gao for technical assistance. W.M. acknowledges support from Columbia University, a US National Institutes of Health Director's New Innovator Award, the US Army Research Office (W911NF-12-1-0594) and an Alfred P. Sloan Research Fellowship. Author Contributions: L.W., F.H., Y.S., Z.C., Y.Y., C.-C.L. and M.C.W. performed experiments and analyzed data. L.W. and W.M. conceived the concept, designed the experiments and wrote the paper. Competing interests: Columbia University, which L.W., F.H., Y.S., Z.C. and W.M. are affiliated with, has filed a patent application based on this work.

Funders:

Funding Agency	Grant Number
Columbia University	UNSPECIFIED
NIH	UNSPECIFIED
Army Research Office (ARO)	W911NF-12-1-0594
Alfred P. Sloan Foundation	UNSPECIFIED

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PubMed Central ID:

PMC4040164

Record Number:

CaltechAUTHORS:20180608-133705699

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20180608-133705699

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No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

86929

Collection:

CaltechAUTHORS

Deposited By:

George Porter

Deposited On:

08 Jun 2018 22:34

Last Modified:

09 Mar 2020 13:19

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