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Postembryonic RNAi in Heterorhabditis bacteriophora: a nematode insect parasite and host for insect pathogenic symbionts

Ciche, Todd A. and Sternberg, Paul W. (2007) Postembryonic RNAi in Heterorhabditis bacteriophora: a nematode insect parasite and host for insect pathogenic symbionts. BMC Developmental Biology, 7 . Art. No. 101. ISSN 1471-213X. PMCID PMC2014770. https://resolver.caltech.edu/CaltechAUTHORS:CICbmcdb07

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Abstract

Background: Heterorhabditis bacteriophora is applied throughout the world for the biological control of insects and is an animal model to study interspecies interactions, e.g. mutualism, parasitism and vector-borne disease. H. bacteriophora nematodes are mutually associated with the insect pathogen, Photorhabdus luminescens. The developmentally arrested infective juvenile (IJ) stage nematode (vector) specifically transmits Photorhabdus luminescens bacteria (pathogen) in its gut mucosa to the haemocoel of insects (host). The nematode vector and pathogen alone are not known to cause insect disease. RNA interference is an excellent reverse genetic tool to study gene function in C. elegans, and it would be useful in H. bacteriophora to exploit the H. bacteriophora genome project, currently in progress. Results: Soaking L1 stage H. bacteriophora with seven dsRNAs of genes whose C. elegans orthologs had severe RNAi phenotypes resulted in highly penetrant and obvious developmental and reproductive abnormalities. The efficacy of postembryonic double strand RNA interference (RNAi) was evident by abnormal gonad morphology and sterility of adult H. bacteriophora and C. elegans presumable due to defects in germ cell proliferation and gonad development. The penetrance of RNAi phenotypes in H. bacteriophora was high for five genes (87-100%; Hba-cct-2, Hba-daf-21, Hba-icd-1; Hba-nol-5, and Hba-W01G7.3) and moderate for two genes (usually 30-50%; Hba-rack-1 and Hba-arf-1). RNAi of three additional C. elegans orthologs for which RNAi phenotypes were not previously detected in C. elegans, also did not result in any apparent phenotypes in H. bacteriophora. Specific and severe reduction in transcript levels in RNAi treated L1s was determined by quantitative real-time RT-PCR. These results suggest that postembryonic RNAi by soaking is potent and specific. Conclusion: Although RNAi is conserved in animals and plants, RNAi using long dsRNA is not. These results demonstrate that RNAi can be used effectively in H. bacteriophora and can be applied for analyses of nematode genes involved in symbiosis and parasitism. It is likely that RNAi will be an important tool for functional genomics utilizing the high quality draft H. bacteriophora genome sequence.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2014770/PubMed CentralArticle
https://doi.org/10.1186/1471-213X-7-101DOIUNSPECIFIED
https://doi.org/10.1186/1471-213X-7-101DOIUNSPECIFIED
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2007 Ciche and Sternberg, licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We graciously thank Prof. Ann Burnell (National University of Ireland-Maynooth) for communicating her then unpublished EST data. T.A. Ciche was supported by a fellowship from the Gordon Ross Foundation. Supported by the Howard Hughes Medical Institute, with which P.W. Sternberg is an Investigator. Authors' contributions: T.A. Ciche designed and performed all experiments and wrote the paper. P.W. Sternberg mentored T.A. Ciche during these experiments by providing valuable advice, laboratory space and resources, and edited the paper. Both authors have read and approved the final version. Accession numbers: The accession numbers (Genbank, dbEST) for H. bacteriophora ESTs used in this study are: EE724174 (Hba-cct-2); EE724171-73 (Hba-daf-21); EE724162, EE724228 (Hba-icd-1); EE724163-64 (Hba-nol-5); EE724167-68 (Hba-W01G7.3); EE724169-70 (Hba-arf-1); EE724158-61, EE724188, EE724199, EE7241206-09 (Hba-rack-1); EE724165-66 (Hba-ben-1); EE724176 (Hba-mrp-4); EE724175 (Hba-nhr-47).
Funders:
Funding AgencyGrant Number
Gordon Ross Medical FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
PubMed Central ID:PMC2014770
Record Number:CaltechAUTHORS:CICbmcdb07
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:CICbmcdb07
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8693
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:06 Sep 2007
Last Modified:02 Oct 2019 23:53

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