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Vibrational Imaging of Glucose Uptake in Live Cells and Tissues by Stimulated Raman Scattering Microscopy

Hu, Fanghao and Chen, Zhixing and Zhang, Luyuan and Shen, Yihui and Wei, Lu and Min, Wei (2015) Vibrational Imaging of Glucose Uptake in Live Cells and Tissues by Stimulated Raman Scattering Microscopy. Biophysical Journal, 108 (2). 480A. ISSN 0006-3495. https://resolver.caltech.edu/CaltechAUTHORS:20180608-135537234

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Abstract

Glucose is a ubiquitous energy source for virtually all living organisms. Its uptake activity closely reflects the cellular metabolic status in various physiological and pathological conditions. Extensive efforts such as positron emission tomography (PET), magnetic resonance imaging (MRI) and fluorescence microscopy have been made to image glucose uptake but all with technical limitations. Here, we report a novel vibrational microscopy platform to visualize glucose uptake in living cells and tissues with subcellular resolution and minimal perturbation by performing stimulated Raman scattering (SRS) on a new glucose analogue. Cancer cells with differing metabolic characteristics can be distinguished. Moreover, heterogeneous glucose uptake patterns are observed with clear cell-cell variations in tumor xenograft tissues as well as in neuronal culture and mouse brain tissues. Therefore, by offering the distinct advantage of optical resolution yet without the undesirable influence of bulky fluorophores, SRS imaging of glucose uptake will be a valuable tool to study energy demands of living systems, particularly in tumors and brain.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.bpj.2014.11.2621DOIArticle
ORCID:
AuthorORCID
Hu, Fanghao0000-0002-8659-4027
Wei, Lu0000-0001-9170-2283
Min, Wei0000-0003-2570-3557
Additional Information:© 2015 Biophysical Society. Published by Elsevier Inc. Available online 27 January 2015. Meeting Abstract: 2420-Pos B557.
Issue or Number:2
Record Number:CaltechAUTHORS:20180608-135537234
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180608-135537234
Official Citation:Fanghao Hu, Zhixing Chen, Luyuan Zhang, Yihui Shen, Lu Wei, Wei Min, Vibrational Imaging of Glucose Uptake in Live Cells and Tissues by Stimulated Raman Scattering Microscopy, Biophysical Journal, Volume 108, Issue 2, Supplement 1, 2015, Page 480a, ISSN 0006-3495, https://doi.org/10.1016/j.bpj.2014.11.2621. (http://www.sciencedirect.com/science/article/pii/S0006349514038338)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86931
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Jun 2018 21:17
Last Modified:23 Oct 2019 20:53

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