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Incorporation of Non-Canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

Fang, Katharine Y. and Lieblich, Seth A. and Tirrell, David A. (2018) Incorporation of Non-Canonical Amino Acids into Proteins by Global Reassignment of Sense Codons. In: Protein Scaffolds. Methods in Molecular Biology. No.1798. Humana Press , New York, NY, pp. 173-186. ISBN 978-1-4939-7892-2.

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Non-canonical amino acids are finding increasing use in basic and applied research. Proteins that evolved naturally for biological function did so by exploiting the chemistries of the canonical amino acids; however, when proteins are repurposed for biomedical and pharmacological applications, they are often subject to conditions different from those characteristic of their original biological environments. Non-canonical amino acids can impart properties that are inaccessible within canonical protein sequence space, and can thereby lead to improved or new functionality. We describe simple methods for global replacement of canonical amino acids by their non-canonical counterparts in recombinant proteins made in high yield in bacterial expression hosts. These methods can be used to engineer both chemical and physical properties of recombinant proteins.

Item Type:Book Section
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URLURL TypeDescription
Lieblich, Seth A.0000-0003-2705-5821
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2018 Springer Science+Business Media, LLC, part of Springer Nature. First Online: 05 June 2018. We thank past and present members of the Tirrell laboratory for their contributions to the development and use of ncAAs in protein science and engineering.
Subject Keywords:Non-canonical amino acids; Protein engineering; Proteomics; Recombinant protein
Series Name:Methods in Molecular Biology
Issue or Number:1798
Record Number:CaltechAUTHORS:20180611-083918324
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:86959
Deposited By: Tony Diaz
Deposited On:11 Jun 2018 20:55
Last Modified:03 Oct 2019 19:50

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