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Enhancer of RNA polymerase III gene transcription

Sturges, Michael R. and Bartilson, Magdalena and Peck, Lawrence J. (1999) Enhancer of RNA polymerase III gene transcription. Nucleic Acids Research, 27 (2). pp. 690-694. ISSN 0305-1048. doi:10.1093/nar/27.2.690.

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A protein responsible for enhanced transcription by RNA polymerase III was identified in extracts from Xenopus oocytes. This protein, called EP3, interacts with a specific DNA sequence adjacent to the 3'-end of a Xenopus somatic 5S RNA gene and forms a distinct band shift complex with a unique DNase I footprint. Enhanced transcription was observed from both 5S RNA and tRNA reporter genes when EP3 binding sites were inserted at different locations and orientations. Removal of the EP3 protein from an oocyte extract abolished this enhanced transcription. In addition, EP3 was shown to stimulate transcription by increasing the rate of transcription complex assembly. EP3 directly discriminates between the somatic and oocyte 5S RNA gene families and may play a significant role in their differential expression during early Xenopus development.

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Additional Information:Copyright © 1999 by Oxford University Press. Received July 20, 1998; Revised October 13, 1998; Accepted November 2, 1998 We would like to thank Mike Fitch for his initial work on EP3, Alissa Lancaster for the construction of the pAL series of plasmids and all the members of the Peck laboratory for their helpful comments and encouragement. The authors also thank Brian Little and Chuck Passavant for their comments on the manuscript. This work was supported by grant GM46232 from the National Institute of General Medical Sciences.
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Deposited On:10 Sep 2007
Last Modified:08 Nov 2021 20:52

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