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Biological consequences of tightly bent DNA: The other life of a macromolecular celebrity

Garcia, Hernan G. and Grayson, Paul and Han, Lin and Inamdar, Mandar and Kondev, Jané and Nelson, Philip C. and Phillips, Rob and Widom, Jonathan and Wiggins, Paul A. (2007) Biological consequences of tightly bent DNA: The other life of a macromolecular celebrity. Biopolymers, 85 (2). pp. 115-130. ISSN 0006-3525. PMCID PMC3496788. doi:10.1002/bip.20627. https://resolver.caltech.edu/CaltechAUTHORS:20180619-104551728

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Abstract

The mechanical properties of DNA play a critical role in many biological functions. For example, DNA packing in viruses involves confining the viral genome in a volume (the viral capsid) with dimensions that are comparable to the DNA persistence length. Similarly, eukaryotic DNA is packed in DNA–protein complexes (nucleosomes), in which DNA is tightly bent around protein spools. DNA is also tightly bent by many proteins that regulate transcription, resulting in a variation in gene expression that is amenable to quantitative analysis. In these cases, DNA loops are formed with lengths that are comparable to or smaller than the DNA persistence length. The aim of this review is to describe the physical forces associated with tightly bent DNA in all of these settings and to explore the biological consequences of such bending, as increasingly accessible by single‐molecule techniques.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/bip.20627 DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3496788PubMed CentralArticle
ORCID:
AuthorORCID
Phillips, Rob0000-0003-3082-2809
Additional Information:© 2006 Wiley Periodicals, Inc. Received 25 October 2006; accepted 26 October 2006; Published online 13 November 2006. We are extremely grateful to a number of people who have given us both guidance and amusement in thinking about these problems (and some for commenting on the manuscript): Sankar Adhya, David Bensimon, Laura Finzi, Bill Gelbart, Jeff Gelles, Uli Gerland, Shura Grosberg, Jack Johnson, Jason Kahn, John Maddocks, Jim Maher, Ian Molineux, Wilma Olson, Prashant Purohit, Michael Rubenstein, Robert Schleif, Andy Spakowitz, Elizabeth Villa, and Nils Walter. RP, PG, LH, MI and PW acknowledge the support of the Keck Foundation, National Science Foundation grant Nos. CMS‐0301657 and CMS‐0404031, and the National Institutes of Health Director's Pioneer Award grant No. DP1 OD000217. HG is grateful for support from both the NSF funded NIRT and the NIH Director's Pioneer Award. JK acknowledges the support of NSF DMR‐0403997 and is a Cottrell Scholar of Research Corporation. PN acknowledges NSF Grant DMR04–04674 and the NSF‐funded NSEC on Molecular Function at the Nano/Bio Interface DMR04–25780. JW acknowledges support from NIH grants R01GM054692 and R01GM058617.
Funders:
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
NSFCMS‐0301657
NSFCMS‐0404031
NIHDP1 OD000217
NSFDMR‐0403997
Research CorporationUNSPECIFIED
NSFDMR04-04674
NSFDMR04-25780
NIHR01GM054692
NIHR01GM058617
Subject Keywords:tightly bent DNA; DNA looping; viral DNA packing; nucleosome accessibility
Issue or Number:2
PubMed Central ID:PMC3496788
DOI:10.1002/bip.20627
Record Number:CaltechAUTHORS:20180619-104551728
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180619-104551728
Official Citation:Garcia, H. G., Grayson, P. , Han, L. , Inamdar, M. , Kondev, J. , Nelson, P. C., Phillips, R. , Widom, J. and Wiggins, P. A. (2007), Biological consequences of tightly bent DNA: The other life of a macromolecular celebrity. Biopolymers, 85: 115-130. doi:10.1002/bip.20627
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87222
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Jun 2018 18:01
Last Modified:15 Nov 2021 20:46

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