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Advanced microscopy to elucidate cardiovascular injury and regeneration: 4D light-sheet imaging

Baek, Kyung In and Ding, Yichen and Chang, Chih-Chiang and Chang, Megan and Sevag Packard, René R. and Hsu, Jeffrey J. and Fei, Peng and Hsiai, Tzung K. (2018) Advanced microscopy to elucidate cardiovascular injury and regeneration: 4D light-sheet imaging. Progress in Biophysics and Molecular Biology, 138 . pp. 105-115. ISSN 0079-6107.

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The advent of 4-dimensional (4D) light-sheet fluorescence microscopy (LSFM) has provided an entry point for rapid image acquisition to uncover real-time cardiovascular structure and function with high axial resolution and minimal photo-bleaching/-toxicity. We hereby review the fundamental principles of our LSFM system to investigate cardiovascular morphogenesis and regeneration after injury. LSFM enables us to reveal the micro-circulation of blood cells in the zebrafish embryo and assess cardiac ventricular remodeling in response to chemotherapy-induced injury using an automated segmentation approach. Next, we review two distinct mechanisms underlying zebrafish vascular regeneration following tail amputation. We elucidate the role of endothelial Notch signaling to restore vascular regeneration after exposure to the redox active ultrafine particles (UFP) in air pollutants. By manipulating the blood viscosity and subsequently, endothelial wall shear stress, we demonstrate the mechanism whereby hemodynamic shear forces impart both mechanical and metabolic effects to modulate vascular regeneration. Overall, the implementation of 4D LSFM allows for the elucidation of mechanisms governing cardiovascular injury and regeneration with high spatiotemporal resolution.

Item Type:Article
Related URLs:
URLURL TypeDescription
Baek, Kyung In0000-0001-9388-2070
Ding, Yichen0000-0002-6242-3506
Sevag Packard, René R.0000-0002-8520-5843
Hsu, Jeffrey J.0000-0002-9971-5916
Hsiai, Tzung K.0000-0003-1734-0792
Additional Information:© 2018 Elsevier Ltd. Received 31 January 2018, Revised 30 April 2018, Accepted 4 May 2018, Available online 9 May 2018.
Subject Keywords:Light-sheet fluorescence microscopy; Cardiovascular injury; Doxorubicin; Vascular regeneration
Record Number:CaltechAUTHORS:20180622-101243466
Persistent URL:
Official Citation:Kyung In Baek, Yichen Ding, Chih-Chiang Chang, Megan Chang, René R. Sevag Packard, Jeffrey J. Hsu, Peng Fei, Tzung K. Hsiai, Advanced microscopy to elucidate cardiovascular injury and regeneration: 4D light-sheet imaging, Progress in Biophysics and Molecular Biology, Volume 138, 2018, Pages 105-115, ISSN 0079-6107, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87320
Deposited By: Tony Diaz
Deposited On:23 Jun 2018 15:35
Last Modified:03 Oct 2019 19:54

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