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Functional epitopes at the ribosome subunit interface

Rackham, Oliver and Wang, Kaihang and Chin, Jason W. (2006) Functional epitopes at the ribosome subunit interface. Nature Chemical Biology, 2 (5). pp. 254-258. ISSN 1552-4450. doi:10.1038/nchembio783.

[img] PDF (Supplementary Table 1 - Library design) - Supplemental Material
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[img] PDF (Supplementary Table 5 - Covariation analysis) - Supplemental Material
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[img] PDF (Supplementary Table 6 - Intersubunit bridge selection data and phylogenetic data) - Supplemental Material
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The ribosome is a 2.5-MDa molecular machine that synthesizes cellular proteins encoded in mRNAs. The 30S and 50S subunits of the ribosome associate through structurally defined intersubunit bridges burying 6,000 Å^2, 80% of which is buried in conserved RNA-RNA interactions. Intersubunit bridges bind translation factors, may coordinate peptide bond formation and translocation and may be actively remodeled in the post-termination complex, but the functional importance of numerous 30S bridge nucleotides had been unknown. We carried out large-scale combinatorial mutagenesis and in vivo selections on 30S nucleotides that form RNA-RNA intersubunit bridges in the Escherichia coli ribosome. We determined the covariation and functional importance of bridge nucleotides, allowing comparison of the structural interface and phylogenetic data to the functional epitope. Our results reveal how information for ribosome function is partitioned across bridges, and suggest a subset of nucleotides that may have measurable effects on individual steps of the translational cycle.

Item Type:Article
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URLURL TypeDescription ReadCube access
Wang, Kaihang0000-0001-7657-8755
Chin, Jason W.0000-0003-1219-4757
Additional Information:© 2006 Macmillan Publishers Limited. Received 2 November 2005; accepted 14 March 2006; published online 2 April 2006. J.W.C. is an EMBO Young Investigator. K.W. is grateful for a Medical Research Council–Laboratory of Molecular Biology (MRC-LMB) Cambridge Scholarship, an Honorary External Research Studentship from Trinity College, Cambridge and an Overseas Research Studentship Award. We thank M.E. Hurles (Sanger Institute) for discussions on quantifying sequence polymorphisms, P.H. Dear (LMB) and G. Mitchison (Cambridge) for discussions on statistical methods, and T.M. Schmeing (LMB) and other members of LMB for critically reading versions of the manuscript. The authors declare no competing financial interests.
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European Molecular Biology Organization (EMBO)UNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Trinity CollegeUNSPECIFIED
Overseas Research Studentship AwardUNSPECIFIED
Issue or Number:5
Record Number:CaltechAUTHORS:20180627-083255769
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87373
Deposited By: Tony Diaz
Deposited On:27 Jun 2018 16:17
Last Modified:15 Nov 2021 20:47

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