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Approaches to distinguishing bacteria from mineral particles in microscopic imaging

Lindensmith, Christian and Bedrossian, Manuel and Nadeau, Jay (2018) Approaches to distinguishing bacteria from mineral particles in microscopic imaging. In: 2018 IEEE Aerospace Conference. IEEE , Piscataway, NJ, pp. 1-9. ISBN 978-1-5386-2014-4. http://resolver.caltech.edu/CaltechAUTHORS:20180706-105934129

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Abstract

Biologists use microscopy as the gold standard for identification of the presence of microbial life in terrestrial environments. Even when DNA analysis is used to identify strains and genetic population diversity, microscopic imaging is typically used to verify the presence of live organisms and obtain information about their sizes and morphologies. With identified microbes, a variety of microscopic techniques, particularly fluorescence imaging, are used for identification of features such as proteins, lipids, and nucleic acids. For identification of microbial life in situ, the challenge is extended to include discrimination of potential live cells from non-living objects of similar size. We present here a survey of analyses that can be made from microscopic images to make this distinction. The techniques include measurement of refractive index, Brownian motion, gravitational settling, autofluorescence, induced fluorescence, and induction of various forms of taxis.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1109/AERO.2018.8396822DOIArticle
ORCID:
AuthorORCID
Nadeau, Jay0000-0001-5258-0076
Additional Information:© 2018 IEEE. The work described in this paper was performed in part at the Jet Propulsion Laboratory, California Institute of Technology under contract with the National Aeronautics and Space Administration and in part at the California Institute of Technology under grants GBMF-4037 and GBMF-4038 from the Gordon and Betty Moore Foundation.
Funders:
Funding AgencyGrant Number
NASA/JPL/CaltechUNSPECIFIED
Gordon and Betty Moore FoundationGBMF-4037
Gordon and Betty Moore FoundationGBMF-4038
Record Number:CaltechAUTHORS:20180706-105934129
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180706-105934129
Official Citation:C. Lindensmith, M. Bedrossian and J. Nadeau, "Approaches to distinguishing bacteria from mineral particles in microscopic imaging IEEE aerospace conference," 2018 IEEE Aerospace Conference, Big Sky, MT, USA, 2018, pp. 1-9. doi: 10.1109/AERO.2018.8396822
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87596
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 Jul 2018 19:38
Last Modified:06 Jul 2018 19:38

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