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Engineering Cell Type Specific Delivery Vectors for Noninvasive Modulation of Brain Circuits and Behaviors

Flytzanis, Nicholas and Goeden, Nicholas and Cho, Jounhong and Kahan, Anat and Luongo, Francisco and Tsao, Doris and Deverman, Benjamin E. and Gradinaru, Viviana (2018) Engineering Cell Type Specific Delivery Vectors for Noninvasive Modulation of Brain Circuits and Behaviors. Molecular Therapy, 26 (5). p. 304. ISSN 1525-0016. http://resolver.caltech.edu/CaltechAUTHORS:20180706-151207096

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Abstract

Within neuroscience the modulation and monitoring of specific circuits is achieved using a combination of transgenic animals and direct injection of viral vectors. To provide an alternative to invasive brain injection and increase transduction coverage and efficiency, we previously engineered adeno-associated viruses (AAVs) that efficiently cross the BBB in adult mice (Deverman et al, 2016; Chan et al, 2017). When delivering genes systemically, however, it is currently not possible to achieve a high degree of specificity for defined neuronal cell types in specific brain regions without the use of transgenic approaches. Here, we attempt to address this limitation by selectively engineering capsids with regional and cell-type specificity using CREATE, the Cre recombination-dependent AAV targeted evolution (Deverman et al, 2016) methodology that recovers capsids that transduce predefined Cre+ target cell populations.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ymthe.2018.05.001DOIAbstracts
ORCID:
AuthorORCID
Flytzanis, Nicholas0000-0002-7921-9392
Deverman, Benjamin E.0000-0002-6223-9303
Gradinaru, Viviana0000-0001-5868-348X
Additional Information:© 2018 American Society of Gene & Cell Therapy. Available online 9 May 2018.
Record Number:CaltechAUTHORS:20180706-151207096
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180706-151207096
Official Citation:ASGCT 21st Annual Meeting Abstracts, Molecular Therapy, Volume 26, Issue 5, Supplement 1, 2018, Pages 1-459, ISSN 1525-0016, https://doi.org/10.1016/j.ymthe.2018.05.001. (http://www.sciencedirect.com/science/article/pii/S1525001618302041)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87616
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Jul 2018 22:25
Last Modified:06 Jul 2018 22:25

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