Chou, Hou-Pu and Spence, Charles and Scherer, Axel and Quake, Stephen (1998) Microfabricated devices for sizing DNA and sorting cells. In: Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications. Proceedings of SPIE. No.3258. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 181-187. ISBN 0819426970. https://resolver.caltech.edu/CaltechAUTHORS:20180710-102548857
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Abstract
We have microfabricated devices to size and sort microscopic biological objects, ranging from cells to single molecules of DNA. Sizing is accomplished by fluorescent excitation and detection. The devices are fabricated in a silicone elastomer using a replica method. Single molecules of DNA have been sized to 10 percent accuracy, and manipulation of E. Coli cells has been demonstrated.
| Item Type: | Book Section | ||||||
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| Additional Information: | © 1998 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank Anne Fu and Frances Arnold for the gift of fluorescent E Coli cells. | ||||||
| Subject Keywords: | MEMS, Cell sorting, DNA diagnostics | ||||||
| Series Name: | Proceedings of SPIE | ||||||
| Issue or Number: | 3258 | ||||||
| DOI: | 10.1117/12.304378 | ||||||
| Record Number: | CaltechAUTHORS:20180710-102548857 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180710-102548857 | ||||||
| Official Citation: | Hou-Pu Chou, Charles Spence, Axel Scherer, Stephen R. Quake, "Microfabricated devices for sizing DNA and sorting cells", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); doi: 10.1117/12.304378; https://doi.org/10.1117/12.304378 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 87694 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | George Porter | ||||||
| Deposited On: | 10 Jul 2018 18:02 | ||||||
| Last Modified: | 15 Nov 2021 20:50 |
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