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Microfabricated devices for sizing DNA and sorting cells

Chou, Hou-Pu and Spence, Charles and Scherer, Axel and Quake, Stephen (1998) Microfabricated devices for sizing DNA and sorting cells. In: Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications. Proceedings of SPIE. No.3258. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 181-187. ISBN 0819426970. https://resolver.caltech.edu/CaltechAUTHORS:20180710-102548857

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Abstract

We have microfabricated devices to size and sort microscopic biological objects, ranging from cells to single molecules of DNA. Sizing is accomplished by fluorescent excitation and detection. The devices are fabricated in a silicone elastomer using a replica method. Single molecules of DNA have been sized to 10 percent accuracy, and manipulation of E. Coli cells has been demonstrated.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.304378DOIArticle
Additional Information:© 1998 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank Anne Fu and Frances Arnold for the gift of fluorescent E Coli cells.
Subject Keywords:MEMS, Cell sorting, DNA diagnostics
Series Name:Proceedings of SPIE
Issue or Number:3258
Record Number:CaltechAUTHORS:20180710-102548857
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180710-102548857
Official Citation:Hou-Pu Chou, Charles Spence, Axel Scherer, Stephen R. Quake, "Microfabricated devices for sizing DNA and sorting cells", Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); doi: 10.1117/12.304378; https://doi.org/10.1117/12.304378
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87694
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Jul 2018 18:02
Last Modified:03 Oct 2019 19:58

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