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Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays

Coppock, Matthew B. and Farrow, Blake and Warner, Candice and Finch, Amethist S. and Lai, Bert and Sarkes, Deborah A. and Heath, James R. and Stratis-Cullum, Dimitra (2014) Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays. In: Smart Biomedical and Physiological Sensor Technology XI. Proceedings of SPIE. No.9107. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. no. 910711. ISBN 9781628410440. https://resolver.caltech.edu/CaltechAUTHORS:20180711-141559479

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Abstract

Current biodetection assays that employ monoclonal antibodies as primary capture agents exhibit limited fieldability, shelf life, and performance due to batch-to-batch production variability and restricted thermal stability. In order to improve upon the detection of biological threats in fieldable assays and systems for the Army, we are investigating protein catalyzed capture (PCC) agents as drop-in replacements for the existing antibody technology through iterative in situ click chemistry. The PCC agent oligopeptides are developed against known protein epitopes and can be mass produced using robotic methods. In this work, a PCC agent under development will be discussed. The performance, including affinity, selectivity, and stability of the capture agent technology, is analyzed by immunoprecipitation, western blotting, and ELISA experiments. The oligopeptide demonstrates superb selectivity coupled with high affinity through multi-ligand design, and improved thermal, chemical, and biochemical stability due to non-natural amino acid PCC agent design


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2052542DOIArticle
ORCID:
AuthorORCID
Heath, James R.0000-0001-5356-4385
Additional Information:© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE). This research was funded primarily provided by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office. Research is supported in part by appointments (M.B.C.) to the U.S. Army Research Laboratory Postdoctoral Fellowship Program administered by the Oak Ridge Associated Universities through a contract with the U.S. Army Research Laboratory. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. The following reagents were obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Anthrax Protective Antigen (PA), recombinant from E. coli, NR-3780.
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-0001
Army Research LaboratoryUNSPECIFIED
Subject Keywords:PCC agent, peptide-based sensors, biological detection, antibody replacements
Series Name:Proceedings of SPIE
Issue or Number:9107
Record Number:CaltechAUTHORS:20180711-141559479
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180711-141559479
Official Citation:Matthew B. Coppock, Blake Farrow, Candice Warner, Amethist S. Finch, Bert Lai, Deborah A. Sarkes, James R. Heath, Dimitra Stratis-Cullum, "Peptide-based protein capture agents with high affinity, selectivity, and stability as antibody replacements in biodetection assays", Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 910711 (22 May 2014); doi: 10.1117/12.2052542; https://doi.org/10.1117/12.2052542
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87766
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jul 2018 15:44
Last Modified:03 Oct 2019 19:59

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