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Feasibility of field-based light scattering spectroscopy

Yang, Changhuei and Perelman, Lev T. and Wax, Adam and Dasari, Ramachandra R. and Feld, Michael S. (2000) Feasibility of field-based light scattering spectroscopy. Journal of Biomedical Optics, 5 (2). pp. 138-143. ISSN 1083-3668. http://resolver.caltech.edu/CaltechAUTHORS:20180711-143825869

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Abstract

Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 mm in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/1.429980DOIArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2000 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper JBO-42014 received Sep. 15, 1999; revised manuscript received Feb. 8, 2000; accepted for publication Mar. 1, 2000. Valuable discussions with Vadim Backman are gratefully acknowledged, as well as help by Rajan Gurjar with various aspects of the experiment. This work was carried out at the MIT Laser Biomedical Research Center and was supported by National Institutes of Health Grant No. P41-RR02594 and a grant from Hamamatsu Corporation.
Funders:
Funding AgencyGrant Number
NIHP41-RR02594
Hamamatsu CorporationUNSPECIFIED
Subject Keywords:light scattering spectroscopy; interferometer; Mie theory
Record Number:CaltechAUTHORS:20180711-143825869
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180711-143825869
Official Citation:Changhuei Yang, Lev T. Perelman, Adam Wax, Ramachandra R. Dasari, Michael S. Feld, "Feasibility of field-based light scattering spectroscopy," Journal of Biomedical Optics 5(2), (1 April 2000). https://doi.org/10.1117/1.429980
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87768
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Jul 2018 23:09
Last Modified:16 Jul 2018 23:09

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