CaltechAUTHORS
  A Caltech Library Service

Generation of functional antigen-specific T cells in defined genetic backgrounds by retrovirus-mediated expression of TCR cDNAs in hematopoietic precursor cells

Yang, Lili and Qin, Xiao-Feng and Baltimore, David and Van Parijs, Luk (2002) Generation of functional antigen-specific T cells in defined genetic backgrounds by retrovirus-mediated expression of TCR cDNAs in hematopoietic precursor cells. Proceedings of the National Academy of Sciences of the United States of America, 99 (9). pp. 6204-6209. ISSN 0027-8424. PMCID PMC122927. https://resolver.caltech.edu/CaltechAUTHORS:YANpnas02

[img]
Preview
PDF - Published Version
See Usage Policy.

264Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:YANpnas02

Abstract

We have developed an alternative to transgenesis for producing antigen-specific T cells in vivo. In this system, clonal naive T cells with defined antigen specificity are generated by retrovirus-mediated expression of T cell antigen receptor cDNAs in RAG1-deficient murine hematopoietic precursor cells. These T cells can be stimulated to proliferate and produce cytokines by exposure to antigen in vitro, and they become activated and expand in vivo after immunization. IL-2-deficient T cells generated by this technique show decreased proliferation and cytokine production, both of which can be rescued by exogenous addition of this growth factor. Thus, retrovirus-mediated expression of T cell antigen receptor cDNAs in hematopoietic precursor cells permits the rapid and efficient analysis of the life history of antigen-specific T cells in different genetic backgrounds and may allow for the long-term production of antigen-specific T cells with different functional properties for prophylactic and therapeutic purposes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1073/pnas.092154599DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc122927/PubMed CentralArticle
ORCID:
AuthorORCID
Baltimore, David0000-0001-8723-8190
Additional Information:© 2002 by the National Academy of Sciences. Contributed by David Baltimore, March 15, 2002. We thank Francis Carbone and William Heath for providing us with the OTII TCR-12 and TCR-12 cDNAs and Nilabh Shastri for providing us with the BWZ cell line. We are grateful to Susan Kovats for her assistance in cell-proliferation assays. X.-F.Q. is the recipient of Damon Runyon–Walter Winchell Fellowship DRG 1568. This work was supported by National Institutes of Health Grants R01 GM39458 (to D.B.) and R21 AI 494897 (to L.V.P.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Damon Runyon-Walter Winchell Cancer FundDRG 1568
NIHR01 GM39458
NIHR21 AI 494897
Issue or Number:9
PubMed Central ID:PMC122927
Record Number:CaltechAUTHORS:YANpnas02
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:YANpnas02
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:878
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Nov 2005
Last Modified:21 Apr 2020 21:38

Repository Staff Only: item control page