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Oligonucleotide that binds nuclear factor NF-kappa-B acts as a lymphoid-specific and inducible enhancer element

Pierce, Jacqueline W. and Lenardo, Michael and Baltimore, David (1988) Oligonucleotide that binds nuclear factor NF-kappa-B acts as a lymphoid-specific and inducible enhancer element. Proceedings of the National Academy of Sciences of the United States of America, 85 (5). pp. 1482-1486. ISSN 0027-8424. doi:10.1073/pnas.85.5.1482.

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The immunoglobulin kappa light chain gene contains a lymphoid-specific enhancer that includes several short protein-binding sequences. The sequence that binds the nuclear factor NF-kappa B was tested for its ability to act independently as an enhancer element by inserting it into test plasmids containing the chloramphenicol acetyltransferase gene. When analyzed for activity by transient transfection into lymphoid and nonlymphoid cells, a single copy of the NF-kappa B binding site could act as a tissue-specific upstream activating element. Two copies (dimer) showed 10-fold higher activity than did one copy and could act as an enhancer element 2.5 kilobases downstream of the transcriptional start site. The enhancer activity of this sequence was correlated with the presence of the cognate binding protein, NF-kappa B. This sequence acted as an inducible enhancer under conditions that induce NF-kappa B binding activity. Thus, the NF-kappa B binding site acts by itself as a tissue-specific and inducible enhancer element, and two copies show cooperative interaction.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1988 by the National Academy of Sciences. Contributed by David Baltimore, November 16, 1987. We thank Michael Paskind for his assistance in sequencing inserted oligonucleotides and Thomas Wirth for useful comments on the manuscript. The work was funded by a Special Fellowship of the Leukemia Society of America to J.W.P., a National Cancer Institute Clinical Investigator Award to M.L., and a grant from the American Cancer Society to D.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Leukemia Society of AmericaUNSPECIFIED
National Cancer InstituteUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Subject Keywords:immunoglobulin kappa light chain gene; transfection
Issue or Number:5
Record Number:CaltechAUTHORS:PIEpnas88
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8785
Deposited By: Tony Diaz
Deposited On:17 Sep 2007
Last Modified:08 Nov 2021 20:53

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