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Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsu, Hsun-Chia and Li, Lei and Yao, Junjie and Wong, Terence T. W. and Shi, Junhui and Chen, Ruimin and Zhou, Qifa and Wang, Lihong V. (2018) Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy. Journal of Biomedical Optics, 23 (7). Art. No. 076001. ISSN 1083-3668. https://resolver.caltech.edu/CaltechAUTHORS:20180716-103911158

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Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated fast, label-free volumetric imaging of optical-absorption contrast within the quasiballistic regime of photon scattering. However, the limited numerical aperture of the ultrasonic transducer restricts the detectability of the photoacoustic waves, thus resulting in incomplete reconstructed features. To tackle the limited-view problem, we added an oblique illumination beam to the original coaxial optical-acoustic scheme to provide a complementary detection view. The virtual augmentation of the detection view was validated through numerical simulations and tissue-phantom experiments. More importantly, the combination of top and oblique illumination successfully imaged a mouse brain in vivo down to 1 mm in depth, showing detailed brain vasculature. Of special note, it clearly revealed the diving vessels that were long missing in images from original OR-PAM.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/1.JBO.23.7.076001DOIArticle
ORCID:
AuthorORCID
Li, Lei0000-0001-6164-2646
Wong, Terence T. W.0000-0001-6399-758X
Shi, Junhui0000-0002-5741-2781
Chen, Ruimin0000-0002-5338-359X
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 180145R received: 9 March 2018; accepted: 8 June 2018; published: 6 July 2018. The authors appreciate Professor James Ballard’s kind help with editing the article. L.W. has a financial interest in Microphotoacoustics, Inc., CalPACT, LLC, and Union Photoacoustic Technologies, Ltd., which, however, did not support this work. This work was supported in part by National Institutes of Health grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), R01 EB016963, U01 NS090579 (BRAIN Initiative), and U01 NS099717 (BRAIN Initiative).
Funders:
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 EB016963
NIHU01 NS090579
NIHU01 NS099717
Subject Keywords:optical-resolution photoacoustic microscopy; limited view phenomenon; oblique illumination; mouse cerebral diving vessels
Issue or Number:7
Record Number:CaltechAUTHORS:20180716-103911158
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180716-103911158
Official Citation:Hsun-Chia Hsu, Lei Li, Junjie Yao, Terence T.W. Wong, Junhui Shi, Ruimin Chen, Qifa Zhou, Lihong V. Wang, "Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy," Journal of Biomedical Optics 23(7), 076001 (6 July 2018). https://doi.org/10.1117/1.JBO.23.7.076001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87875
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Jul 2018 17:59
Last Modified:09 Mar 2020 13:19

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