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Quantitative phase imaging using grating-based quadrature phase interferometer

Wu, Jigang and Yaqoob, Zahid and Heng, Xin and Cui, Xiquan and Yang, Changhuei (2007) Quantitative phase imaging using grating-based quadrature phase interferometer. In: Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV. Proceedings of SPIE. No.6443. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 64430J. ISBN 9780819465566. https://resolver.caltech.edu/CaltechAUTHORS:20180716-111909823

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Abstract

In this paper, we report the use of holographic gratings, which act as the free-space equivalent of the 3x3 fiber-optic coupler, to perform full field phase imaging. By recording two harmonically-related gratings in the same holographic plate, we are able to obtain nontrivial phase shift between different output ports of the gratings-based Mach-Zehnder interferometer. The phase difference can be adjusted by changing the relative phase of the recording beams when recording the hologram. We have built a Mach-Zehnder interferometer using harmonically-related holographic gratings with 600 and 1200 lines/mm spacing. Two CCD cameras at the output ports of the gratings-based Mach-Zehnder interferometer are used to record the full-field quadrature interferograms, which are subsequently processed to reconstruct the phase image. The imaging system has ~12X magnification with ~420μmx315μm field-of-view. To demonstrate the capability of our system, we have successfully performed phase imaging of a pure phase object and a paramecium caudatum.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.701694DOIArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2007 Society of Photo-Optical Instrumentation Engineers (SPIE).
Subject Keywords:Diffraction gratings, quadrature phase interferometry, medical and biological imaging
Series Name:Proceedings of SPIE
Issue or Number:6443
Record Number:CaltechAUTHORS:20180716-111909823
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180716-111909823
Official Citation:Jigang Wu, Zahid Yaqoob, Xin Heng, Xiquan Cui, Changhuei Yang, "Quantitative phase imaging using grating-based quadrature phase interferometer", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430J (14 February 2007); doi: 10.1117/12.701694; https://doi.org/10.1117/12.701694
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87883
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:16 Jul 2018 22:41
Last Modified:03 Oct 2019 20:00

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