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Optofluidic microscope and its applications in biology

Heng, Xin and Reynolds, Kevin W. and Cui, Xiquan and Erickson, David and Psaltis, Demetri and Yang, Changhuei (2006) Optofluidic microscope and its applications in biology. In: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV. Proceedings of SPIE. No.6088. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 608816. ISBN 081946130X.

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In this article, we will demonstrate a novel optical imaging device that can be directly integrated into a microfluidic network, and therefore enables on-chip imaging in a microfluidic system. This micro imaging device, termed optofluidic microscope (OFM) is potentially free of bulk optics and is based on a nanohole array defined in a nontransmissive metallic layer that is patterned onto the floor of the microfluidic channel. The operation of the optofluidic microscope will be explained in details and its performance is examined by using a popular animal model, Caenorhabditis elegans (C. elegans). Images from a large population of nematode worms are efficiently acquired within a short time frame. The quality of the OFM images of C. elegans and the morphological characteristics revealed therein are evaluated. Two groups of early-stage C elegans larvae, wild-type and dpy-24 are successfully separated even though their morphological difference at the larval stage is subtle. The experimental results support our claim that the methodology described therein can be effectively used to develop a powerful tool for fulfilling high-resolution, high-throughput imaging task in microfluidics-based systems.

Item Type:Book Section
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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2006 Society of Photo-Optical Instrumentation Engineers (SPIE). We are grateful for the generous help from Dr. Larry R. Baugh and Professor Sternberg regarding C elegans culturing and phenotyping, and the help from Professor Axel Scherer, Dr. Joerg Schilling in fabrication. We thank Dr. Zahid Yaqoob, Jigang Wu for constructive discussions. The assistance from Cornell Nanofabrication Facility and Caltech Watson cleanroom is appreciated. This project is funded by DARPA’s center for optofluidic integration.
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Subject Keywords:Microfluidics, Nanophootnics, C elegans phenotyping
Series Name:Proceedings of SPIE
Issue or Number:6088
Record Number:CaltechAUTHORS:20180716-160347524
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Official Citation:Xin Heng, Kevin W. Reynolds, Xiquan Cui, David Erickson, Demetri Psaltis, Changhuei Yang, "Optofluidic microscope and its applications in biology", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608816 (21 February 2006); doi: 10.1117/12.640325;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87895
Deposited By: George Porter
Deposited On:16 Jul 2018 23:13
Last Modified:03 Oct 2019 20:00

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