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Pump-probe optical coherence tomography using indocyanine green as a contrast agent

Yaqoob, Zahid and McDowell, Emily and Wu, Jigang and Yang, Changhuei (2006) Pump-probe optical coherence tomography using indocyanine green as a contrast agent. In: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X. Proceedings of SPIE. No.6079. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 607904. ISBN 0819461210. http://resolver.caltech.edu/CaltechAUTHORS:20180716-162002796

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Abstract

Use of indocyanine green (ICG), an FDA-approved dye, in a pump-probe scheme for optical coherence tomography (OCT) is reported. Aqueous solutions of ICG are not stable, i.e., the dye degrades over time especially in the presence of light. Addition of protein such as bovine serum albumin (BSA) stabilizes the ICG; however, when exposed to high intensity illumination, the dye still degrades. Moreover, the photodegradation is permanent and occurs swiftly if the illumination band corresponds to the ICG absorption peak. The permanence of the photobleached state illustrates that ICG photobleaching phenomenon has great potential to achieve contrast in OCT. ICG solutions with 50 micromolar concentration were prepared in water, 1% BSA, and 0.8% agarose to study the dynamics of the dye for different illumination intensity levels. In addition, different molar concentrations of ICG in water were studied for fixed illumination intensity. In each case, probability of photobleaching, defined as the ratio of the total photobleached ICG molecules to the total photons absorbed by the ground-state molecules, is evaluated to characterize the photobleaching phenomenon in ICG. We also demonstrate ICG-based pump-probe MCOCT imaging by mapping the distribution of ICG in a stage 54 Xenopus laevis.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.646509DOIArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2006 Society of Photo-Optical Instrumentation Engineers (SPIE). This research was supported by National Institute of Health (NIH) grant 1RZ1EB004602-1.
Funders:
Funding AgencyGrant Number
NIH1RZ1EB004602-1
Subject Keywords:Optical coherence tomography, Indocyanine green, Medical and biological imaging, Pump-probe
Record Number:CaltechAUTHORS:20180716-162002796
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180716-162002796
Official Citation:Zahid Yaqoob, Emily McDowell, Jigang Wu, Changhuei Yang, "Pump-probe optical coherence tomography using indocyanine green as a contrast agent", Proc. SPIE 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X, 607904 (20 February 2006); doi: 10.1117/12.646509; https://doi.org/10.1117/12.646509
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87898
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:17 Jul 2018 15:21
Last Modified:17 Jul 2018 15:21

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