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A new imaging method: optofluidic microscopy

Heng, Xin and Erickson, David and Psaltis, Demetri and Yang, Changhuei (2005) A new imaging method: optofluidic microscopy. In: Nanostructure Integration Techniques for Manufacturable Devices, Circuits, and Systems: Interfaces, Interconnects, and Nanosystems. Proceedings of SPIE. No.6003. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 60030F. ISBN 0819460273.

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In this work, we present a novel optical imaging device that can be directly integrated into a microfluidic network, and can therefore enable on-chip imaging in a microfluidic system. This micro imaging device, termed optofluidic microscope (OFM) is free of bulk optics and is based on a nanohole array defined in a non-transmissive metallic layer that is patterned onto the floor of the microfluidic channel. The operation of the optofluidic microscope is explained in details and its performance is examined with Caenorhabditis elegans (C. elegans) of various genotypes. Images from a large population of worms have been efficiently acquired within a short time frame. The quality of the OFM images of C elegans and the morphological characteristics revealed by the images are evaluated. The experimental results support our claim that the methodology described therein promises to create a powerful tool for fulfilling high- resolution, high-throughput imaging task of the microscopic biological samples.

Item Type:Book Section
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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2005 Society of Photo-Optical Instrumentation Engineers (SPIE). We are grateful for the generous help from Dr. Larry R. Baugh and Professor Sternberg regarding C elegans culturing and phenotyping, and from Professor Axel Scherer, Dr. Joerg Schilling in fabrication. We thank Dr. Zahid Yaqoob, Kevin Reynolds, Xiquan Cui for constructive discussions. The assistance from Cornell Nanofabrication Facility and Caltech Watson cleanroom is well appreciated. This project is funded by DARPA’s center for optofluidic integration.
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Subject Keywords:Nanophotonics, Microfluidics, C elegans phenotyping
Series Name:Proceedings of SPIE
Issue or Number:6003
Record Number:CaltechAUTHORS:20180716-162340521
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Official Citation:Xin Heng, David Erickson, Demetri Psaltis, Changhuei Yang, "A new imaging method: optofluidic microscopy", Proc. SPIE 6003, Nanostructure Integration Techniques for Manufacturable Devices, Circuits, and Systems: Interfaces, Interconnects, and Nanosystems, 60030F (12 November 2005); doi: 10.1117/12.632157;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87899
Deposited By: George Porter
Deposited On:17 Jul 2018 15:06
Last Modified:03 Oct 2019 20:01

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