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Molecular contrast optical coherence tomography: SNR comparison of techniques and introduction of ground state recovery pump-probe OCT

Applegate, Brian E. and Sarunic, Marinko V. and Yang, Changhuei and Izatt, Joseph A. (2005) Molecular contrast optical coherence tomography: SNR comparison of techniques and introduction of ground state recovery pump-probe OCT. In: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX. Proceedings of SPIE. No.5690. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 182-186. ISBN 0819456640. http://resolver.caltech.edu/CaltechAUTHORS:20180716-164319113

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Abstract

Molecular contrast OCT (MCOCT) is an extension of OCT in which specific molecular species are imaged based on their spectroscopic characteristics. In order to improve the sensitivity and specificity of MCOCT, several techniques have recently been introduced which depend upon coherent detection of inelastically scattered light from molecules of interest in a sample. These techniques include harmonic generation, coherent anti-Stokes Raman scattering, and several different forms of pump-probe spectroscopy. We have developed a theoretical framework to facilitate the comparison of different inelastic scattering-based contrast mechanisms for molecular contrast OCT. This framework is based upon the observation that since the noise floor is defined by the reference arm power in a shot-noise limited heterodyne detection system, the relevant comparison among the techniques is isolated to the available molecular-specific signal power. We have derived the value of the molecular contrast signal power for second harmonic generation OCT (SHOCT) and three different pump-probe OCT (PPOCT) techniques. Motivated by this analysis, we have constructed a preliminary ground state recovery pump-probe OCT system, and demonstrated its performance using rhodamine 6G as the MCOCT contrast agent.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.592866DOIArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2005 Society of Photo-Optical Instrumentation Engineers (SPIE). We gratefully acknowledge support for this work through a grant from the National Institute of Health, R01 EB000243. BEA acknowledges support via a Kirschstein postdoctoral fellowship, F32 EB004237.
Funders:
Funding AgencyGrant Number
NIHR01 EB000243
NIH Postdoctoral FellowshipF32 EB004237
Subject Keywords:molecular contrast, optical coherence tomography, molecular imaging, nonlinear microscopy
Record Number:CaltechAUTHORS:20180716-164319113
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180716-164319113
Official Citation:Brian E. Applegate, Marinko V. Sarunic, Changhuei Yang, Joseph A. Izatt, "Molecular contrast optical coherence tomography: SNR comparison of techniques and introduction of ground state recovery pump-probe OCT", Proc. SPIE 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, (13 April 2005); doi: 10.1117/12.592866; https://doi.org/10.1117/12.592866
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87902
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:17 Jul 2018 15:43
Last Modified:17 Jul 2018 15:43

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