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A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices

Rogers, Crystal D. and Sorrells, Lisa K. and Bronner, Marianne E. (2018) A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices. Mechanisms of Development, 152 . pp. 44-56. ISSN 0925-4773. PMCID PMC6112866. https://resolver.caltech.edu/CaltechAUTHORS:20180717-154246397

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Abstract

Characterizing endogenous protein expression, interaction and function, this study identifies in vivo interactions and competitive balance between N-cadherin and E-cadherin in developing avian (Gallus gallus) neural and neural crest cells. Numerous cadherin proteins, including neural cadherin (Ncad) and epithelial cadherin (Ecad), are expressed in the developing neural plate as well as in neural crest cells as they delaminate from the newly closed neural tube. To clarify independent or coordinate function during development, we examined their expression in the cranial region. The results revealed surprising overlap and distinct localization of Ecad and Ncad in the neural tube. Using a proximity ligation assay and co-immunoprecipitation, we found that Ncad and Ecad formed heterotypic complexes in the developing neural tube, and that modulation of Ncad levels led to reciprocal gain or reduction of Ecad protein, which then alters ectodermal cell fate. Here, we demonstrate that the balance of Ecad and Ncad is dependent upon the availability of β-catenin proteins, and that alteration of either classical cadherin modifies the proportions of the neural crest and neuroectodermal cells that are specified.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.mod.2018.07.003DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112866PubMed CentralArticle
ORCID:
AuthorORCID
Rogers, Crystal D.0000-0002-9549-1089
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2018 Published by Elsevier B.V. Received 12 April 2018, Revised 16 June 2018, Accepted 2 July 2018, Available online 14 July 2018. We would like to thank Dr. Mary-Pat Stein at CSUN and the Bronner lab at Caltech for helpful discussions, the CSUN biology department for funding and assistance, and the NIH NICHD for funding. Special thanks to Jackie Phillips and Karla Terrazas for their assistance with this project and for being super students and super women. No competing interests declared. Author contributions: Conceptualization, CDR and MEB; Methodology, CDR; Investigation, CDR and LKS; Validation and Formal Analysis, CDR; Writing –Original Draft, CDR; Writing –Review & Editing, CDR and MEB; Visualization, CDR; Funding Acquisition, CDR and MEB; Resources, CDR and MEB; Supervision, CDR and MEB. This work was supported by a National Institute of Health, NICHD P01 grant HD037105 to MEB, Startup Funding from CSUN to CDR, and a National Institute of Health, NICHD R15 grant R15HD092170 to CDR.
Funders:
Funding AgencyGrant Number
California State University, NorthridgeUNSPECIFIED
NIHHD037105
NIHR15HD092170
Subject Keywords:E-cadherin; N-cadherin; Neural crest; Neuroectoderm; Specification; Proliferation; Cell fate; β-Catenin
PubMed Central ID:PMC6112866
Record Number:CaltechAUTHORS:20180717-154246397
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180717-154246397
Official Citation:Crystal D. Rogers, Lisa K. Sorrells, Marianne E. Bronner, A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices, Mechanisms of Development, Volume 152, 2018, Pages 44-56, ISSN 0925-4773, https://doi.org/10.1016/j.mod.2018.07.003. (http://www.sciencedirect.com/science/article/pii/S0925477318300819)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:87944
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Jul 2018 22:55
Last Modified:03 Oct 2019 20:01

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