CaltechAUTHORS
  A Caltech Library Service

New implementation of second harmonic generation microscopy for three-dimensional resolution

Yang, Changhuei and Mertz, Jerome (2003) New implementation of second harmonic generation microscopy for three-dimensional resolution. In: Multiphoton Microscopy in the Biomedical Sciences III. Proceedings of SPIE. No.4963. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 52-59. ISBN 0819447633. https://resolver.caltech.edu/CaltechAUTHORS:20180719-111608529

[img] PDF - Published Version
See Usage Policy.

256Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20180719-111608529

Abstract

We present a fast scanning transmission-mode confocal scanning laser microscope system based on the use of a second harmonic generation (SHG) crystal for signal detection. The quadratic intensity dependence of SHG is exploited to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal plays the role of a virtual pinhole that remains self-aligned without a need for de-scanning. We demonstrate that this new microscope method produces images with higher contrast and less speckle than transmission scanning microscopy with linear detection.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.478032DOIArticle
ORCID:
AuthorORCID
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2003 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank L. Moreaux for initial work on this project. C. Y. is grateful to INSERM (France) and ASTAR (Singapore) for financial support and fellowship award.
Funders:
Funding AgencyGrant Number
Institut national de la santé et de la recherche médicale (INSERM)UNSPECIFIED
Agency for Science, Technology and Research (A*STAR)UNSPECIFIED
Subject Keywords:second harmonic, confocal, microscopy
Series Name:Proceedings of SPIE
Issue or Number:4963
Record Number:CaltechAUTHORS:20180719-111608529
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180719-111608529
Official Citation:Changhuei Yang, Jerome Mertz, "New implementation of second harmonic generation microscopy for three-dimensional resolution", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478032; https://doi.org/10.1117/12.478032
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88000
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jul 2018 20:04
Last Modified:03 Oct 2019 20:01

Repository Staff Only: item control page