Yang, Changhuei and Mertz, Jerome (2003) New implementation of second harmonic generation microscopy for three-dimensional resolution. In: Multiphoton Microscopy in the Biomedical Sciences III. Proceedings of SPIE. No.4963. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, pp. 52-59. ISBN 0819447633. https://resolver.caltech.edu/CaltechAUTHORS:20180719-111608529
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Abstract
We present a fast scanning transmission-mode confocal scanning laser microscope system based on the use of a second harmonic generation (SHG) crystal for signal detection. The quadratic intensity dependence of SHG is exploited to preferentially reveal unscattered signal light and reject out-of-focus scattered background. The SHG crystal plays the role of a virtual pinhole that remains self-aligned without a need for de-scanning. We demonstrate that this new microscope method produces images with higher contrast and less speckle than transmission scanning microscopy with linear detection.
| Item Type: | Book Section | ||||||
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| Additional Information: | © 2003 Society of Photo-Optical Instrumentation Engineers (SPIE). We thank L. Moreaux for initial work on this project. C. Y. is grateful to INSERM (France) and ASTAR (Singapore) for financial support and fellowship award. | ||||||
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| Subject Keywords: | second harmonic, confocal, microscopy | ||||||
| Series Name: | Proceedings of SPIE | ||||||
| Issue or Number: | 4963 | ||||||
| DOI: | 10.1117/12.478032 | ||||||
| Record Number: | CaltechAUTHORS:20180719-111608529 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180719-111608529 | ||||||
| Official Citation: | Changhuei Yang, Jerome Mertz, "New implementation of second harmonic generation microscopy for three-dimensional resolution", Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478032; https://doi.org/10.1117/12.478032 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 88000 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | George Porter | ||||||
| Deposited On: | 19 Jul 2018 20:04 | ||||||
| Last Modified: | 16 Nov 2021 00:22 |
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