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Two‐dimensional electrophoresis of proteins as a tool in the metabolic engineering of cell cycle regulation

Lee, Kelvin H. and Harrington, Michael G. and Bailey, James E. (1996) Two‐dimensional electrophoresis of proteins as a tool in the metabolic engineering of cell cycle regulation. Biotechnology and Bioengineering, 50 (3). pp. 336-340. ISSN 0006-3592. http://resolver.caltech.edu/CaltechAUTHORS:20180719-155150039

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Abstract

Metabolic engineering of cell cycle regulation addresses important biotechnological problems about serum removal from animal cell culture systems. Chinese hamster ovary cells stimulated to grow by fetal calf serum, insulin, or basic fibroblast growth factor were studied by two‐dimensional electrophoresis (2DE) and the resulting protein expression patterns were analyzed. Detailed 2DE protocols are provided and at least 24 gene products are identified which may play an important role in growth factor signaling. Moreover, a correlation between the expression of three proteins (cyclin D1, cyclin E, and E2F‐1) and mitogenic strength was found.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/(SICI)1097-0290(19960505)50:3%3C336::AID-BIT12%3E3.0.CO;2-LDOIArticle
ORCID:
AuthorORCID
Harrington, Michael G.0000-0002-7923-8032
Additional Information:© 1996 John Wiley & Sons. Received July 11, 1995; Accepted December 7, 1995. The authors thank Bengt Bjellqvist, Wolfgang Renner, Adriana Sburlati, Cornelia Schwerdel, and Christian Weikert for useful discussions and technical assistance. This work was funded by the Swiss Priority Program in Biotechnology Grant No. 5002-037031.
Funders:
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)5002-037031
Subject Keywords:cell cycle; metabolic engineering; two-dimensional electrophoresis of proteins; CHO cell
Record Number:CaltechAUTHORS:20180719-155150039
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180719-155150039
Official Citation:Lee, K. H., Harrington, M. G. and Bailey, J. E. (1996), Two‐dimensional electrophoresis of proteins as a tool in the metabolic engineering of cell cycle regulation. Biotechnol. Bioeng., 50: 336-340. doi:10.1002/(SICI)1097-0290(19960505)50:3<336::AID-BIT12>3.0.CO;2-L
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88034
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jul 2018 23:03
Last Modified:19 Jul 2018 23:03

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